Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Barley includes semi-dwarf varieties, called uzu, which are localized in parts of southwestern Japan, the southern Korea peninsula, and coastal areas of China. The uzu phenotype possesses dark green leaves and short coleoptiles, awns, and panicles. It is controlled by a single recessive gene: uzu. Uzu results from a mutation in the brassinosteroid receptor kinase gene (HvBRI1). Brassinosteroid synergistically acts with auxin on plant morphology, which is an important plant hormone for tissue culture. For this study, tissue culture traits, including callus growth and shoot regeneration capability, were examined in F2 populations derived from crosses between normal and uzu lines, and in isogenic lines for the uzu gene. The uzu genotype shows a lower percentage of shoot regeneration than the normal genotype in F2 populations and isogenic lines. The uzu gene negatively affects shoot regeneration. No significant differences were found in callus growth capability between uzu and normal genotypes. Uzu isogenic lines show higher sensitivity to exogenous auxin for callus initiation than normal lines, when immature embryos were incubated on media supplemented with several concentrations of 2,4-D under culture at higher temperature (25°C). Tissue culture traits of uzu might be regulated through cross-talk between brassinosteroid and auxin.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalBreeding Science
Volume58
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Brassinosteroids
Plant Genes
Indoleacetic Acids
brassinosteroids
Bony Callus
isogenic lines
Hordeum
embryo (plant)
tissue culture
Regeneration
auxins
callus
Embryonic Structures
immatures
barley
Genotype
shoots
genotype
dwarf cultivars
Genes

Keywords

  • Auxin
  • Barley
  • Brassinosteroid
  • Callus formation
  • Hordeum vulgare L.
  • Plant regeneration
  • Uzu

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Uzu, a barley semi-dwarf gene, suppresses plant regeneration in calli derived from immature embryos. / Rikiishi, Kazuhide; Saisho, Daisuke; Takeda, Kazuyoshi.

In: Breeding Science, Vol. 58, No. 2, 2008, p. 149-155.

Research output: Contribution to journalArticle

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