UVB radiation and 17-hydroxygeranyllinalool diterpene glycosides provide durable resistance against mirid (Tupiocoris notatus) attack in field-grown Nicotiana attenuata plants

Son TruòNg DInh, Ivan Galis, Ian T. Baldwin

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Depending on geographical location, plants are exposed to variable amounts of UVB radiation and herbivore attack. Because the role(s) of UVB in the priming and/or accumulation of plant defence metabolites against herbivores are not well understood, we used field-grown Nicotiana attenuata plants to explore the effects of UVB on herbivore performance. Consistent with previous reports, UVB-exposed plants accumulated higher levels of ultraviolet (UV)-absorbing compounds (rutin, chlorogenic acid, crypto-chlorogenic acid and dicaffeoylspermidine). Furthermore, UVB increased the accumulation of jasmonic acid, jasmonoyl-L-isoleucine and abscisic acid, all phytohormones which regulate plant defence against biotic and abiotic stress. In herbivore bioassays, N.attenuata plants experimentally protected from UVB were more infested by mirids in three consecutive field seasons. Among defence metabolites measured, 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs) showed strongly altered accumulation patterns. While constitutive HGL-DTGs levels were higher under UVB, N.attenuata plants exposed to mirid bugs (Tupiocoris notatus) had still more HGL-DTGs under UVB, and mirids preferred to feed on HGL-DTGs-silenced plants when other UVB protecting factors were eliminated by UVB filters. We conclude that UVB exposure not only stimulates UV protective screens but also affects plant defence mechanisms, such as HGL-DTGs accumulation, and modulates ecological interactions of N.attenuata with its herbivores in nature. The ecological interaction of Nicotiana attenuata and Tupiocoris notatus under natural and attenuated UVB fluences in the field was studied. N. attenuata plants accumulated more HGL-DTGs which could function not only in UVB resistance but also as defenses against T. notatus, an abundant herbivore in the plant's natural habitat. This study contributes to our knowledge of solar UVB effects on terrestrial plants and their ecological interactions.

Original languageEnglish
Pages (from-to)590-606
Number of pages17
JournalPlant, Cell and Environment
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Tupiocoris
Diterpenes
Glycosides
diterpenoids
Tobacco
glycosides
Radiation
Herbivory
herbivores
Chlorogenic Acid
chlorogenic acid
Nicotiana attenuata
metabolites
Rutin
Abscisic Acid
Plant Growth Regulators
Isoleucine
Geographical Locations
Defense Mechanisms
isoleucine

Keywords

  • 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs)
  • Manduca sexta (tobacco hornworm)
  • N.attenuata
  • Plant-herbivore interactions
  • Tupiocoris notatus (mirid bug)

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

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title = "UVB radiation and 17-hydroxygeranyllinalool diterpene glycosides provide durable resistance against mirid (Tupiocoris notatus) attack in field-grown Nicotiana attenuata plants",
abstract = "Depending on geographical location, plants are exposed to variable amounts of UVB radiation and herbivore attack. Because the role(s) of UVB in the priming and/or accumulation of plant defence metabolites against herbivores are not well understood, we used field-grown Nicotiana attenuata plants to explore the effects of UVB on herbivore performance. Consistent with previous reports, UVB-exposed plants accumulated higher levels of ultraviolet (UV)-absorbing compounds (rutin, chlorogenic acid, crypto-chlorogenic acid and dicaffeoylspermidine). Furthermore, UVB increased the accumulation of jasmonic acid, jasmonoyl-L-isoleucine and abscisic acid, all phytohormones which regulate plant defence against biotic and abiotic stress. In herbivore bioassays, N.attenuata plants experimentally protected from UVB were more infested by mirids in three consecutive field seasons. Among defence metabolites measured, 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs) showed strongly altered accumulation patterns. While constitutive HGL-DTGs levels were higher under UVB, N.attenuata plants exposed to mirid bugs (Tupiocoris notatus) had still more HGL-DTGs under UVB, and mirids preferred to feed on HGL-DTGs-silenced plants when other UVB protecting factors were eliminated by UVB filters. We conclude that UVB exposure not only stimulates UV protective screens but also affects plant defence mechanisms, such as HGL-DTGs accumulation, and modulates ecological interactions of N.attenuata with its herbivores in nature. The ecological interaction of Nicotiana attenuata and Tupiocoris notatus under natural and attenuated UVB fluences in the field was studied. N. attenuata plants accumulated more HGL-DTGs which could function not only in UVB resistance but also as defenses against T. notatus, an abundant herbivore in the plant's natural habitat. This study contributes to our knowledge of solar UVB effects on terrestrial plants and their ecological interactions.",
keywords = "17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs), Manduca sexta (tobacco hornworm), N.attenuata, Plant-herbivore interactions, Tupiocoris notatus (mirid bug)",
author = "DInh, {Son Tru{\`o}Ng} and Ivan Galis and Baldwin, {Ian T.}",
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T1 - UVB radiation and 17-hydroxygeranyllinalool diterpene glycosides provide durable resistance against mirid (Tupiocoris notatus) attack in field-grown Nicotiana attenuata plants

AU - DInh, Son TruòNg

AU - Galis, Ivan

AU - Baldwin, Ian T.

PY - 2013/3

Y1 - 2013/3

N2 - Depending on geographical location, plants are exposed to variable amounts of UVB radiation and herbivore attack. Because the role(s) of UVB in the priming and/or accumulation of plant defence metabolites against herbivores are not well understood, we used field-grown Nicotiana attenuata plants to explore the effects of UVB on herbivore performance. Consistent with previous reports, UVB-exposed plants accumulated higher levels of ultraviolet (UV)-absorbing compounds (rutin, chlorogenic acid, crypto-chlorogenic acid and dicaffeoylspermidine). Furthermore, UVB increased the accumulation of jasmonic acid, jasmonoyl-L-isoleucine and abscisic acid, all phytohormones which regulate plant defence against biotic and abiotic stress. In herbivore bioassays, N.attenuata plants experimentally protected from UVB were more infested by mirids in three consecutive field seasons. Among defence metabolites measured, 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs) showed strongly altered accumulation patterns. While constitutive HGL-DTGs levels were higher under UVB, N.attenuata plants exposed to mirid bugs (Tupiocoris notatus) had still more HGL-DTGs under UVB, and mirids preferred to feed on HGL-DTGs-silenced plants when other UVB protecting factors were eliminated by UVB filters. We conclude that UVB exposure not only stimulates UV protective screens but also affects plant defence mechanisms, such as HGL-DTGs accumulation, and modulates ecological interactions of N.attenuata with its herbivores in nature. The ecological interaction of Nicotiana attenuata and Tupiocoris notatus under natural and attenuated UVB fluences in the field was studied. N. attenuata plants accumulated more HGL-DTGs which could function not only in UVB resistance but also as defenses against T. notatus, an abundant herbivore in the plant's natural habitat. This study contributes to our knowledge of solar UVB effects on terrestrial plants and their ecological interactions.

AB - Depending on geographical location, plants are exposed to variable amounts of UVB radiation and herbivore attack. Because the role(s) of UVB in the priming and/or accumulation of plant defence metabolites against herbivores are not well understood, we used field-grown Nicotiana attenuata plants to explore the effects of UVB on herbivore performance. Consistent with previous reports, UVB-exposed plants accumulated higher levels of ultraviolet (UV)-absorbing compounds (rutin, chlorogenic acid, crypto-chlorogenic acid and dicaffeoylspermidine). Furthermore, UVB increased the accumulation of jasmonic acid, jasmonoyl-L-isoleucine and abscisic acid, all phytohormones which regulate plant defence against biotic and abiotic stress. In herbivore bioassays, N.attenuata plants experimentally protected from UVB were more infested by mirids in three consecutive field seasons. Among defence metabolites measured, 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs) showed strongly altered accumulation patterns. While constitutive HGL-DTGs levels were higher under UVB, N.attenuata plants exposed to mirid bugs (Tupiocoris notatus) had still more HGL-DTGs under UVB, and mirids preferred to feed on HGL-DTGs-silenced plants when other UVB protecting factors were eliminated by UVB filters. We conclude that UVB exposure not only stimulates UV protective screens but also affects plant defence mechanisms, such as HGL-DTGs accumulation, and modulates ecological interactions of N.attenuata with its herbivores in nature. The ecological interaction of Nicotiana attenuata and Tupiocoris notatus under natural and attenuated UVB fluences in the field was studied. N. attenuata plants accumulated more HGL-DTGs which could function not only in UVB resistance but also as defenses against T. notatus, an abundant herbivore in the plant's natural habitat. This study contributes to our knowledge of solar UVB effects on terrestrial plants and their ecological interactions.

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KW - Manduca sexta (tobacco hornworm)

KW - N.attenuata

KW - Plant-herbivore interactions

KW - Tupiocoris notatus (mirid bug)

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