Utilization of liposomes in vesicular transport studies

Masahiro Kinuta, Kohji Takei

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

Various coated vesicles are implicated in the intracellular transport between different compartments. In vitro reconstitution is a powerful experimental system to study molecular mechanisms involved in assembly of coat proteins from cytosol onto membranes as well as formation of coated vesicles. Liposomes have been recently utilized in the cell-free systems. In this review, we summarize studies on reconstitutions of coated vesicles or coated structures on liposomes. A novel method using dynamic light scattering (DLS) to quantify vesicle formation from liposomes also is described. Our recent study on the role of phospholipids in vesicle formation, where the DSL assay is used in combination with lipid analysis, also is introduced.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalCell structure and function
Volume27
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • Coat proteins
  • Endocytosis
  • Ligt scattering
  • Liposome
  • Phospholipids
  • Vesicular transport

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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