Using CD40-activated B cells to efficiently identify epitopes of tumor antigens

Eisei Kondo, Luise Gryschok, Joachim L. Schultze, Michael S. Von Bergwelt-Baildon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The rapid development of genomics and proteomics has accelerated the discovery of antigens that play a role in host-tumor interaction and can be potentially targeted in tumor immunotherapy. Several independent approaches to characterize such antigens and identify the relevant epitopes have been developed. However, the detection, expansion, and characterization of antigen-specific T cells are essential steps common to all strategies. Efficient identification of epitopes, in particular in a preclinical setting, is often hampered by lack of significant numbers of antigen presenting cells at sufficient purity that readily expand low-frequency T-cell precursors. Using the cylins as a model family of self-tumor antigens, we show that CD40-activated primary human B cells can be used very efficiently to identify novel epitopes and characterize such tumor antigen-specific T cells.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalJournal of Immunotherapy
Volume32
Issue number2
DOIs
Publication statusPublished - Feb 1 2009

Keywords

  • Antigen presenting cell
  • Epitope
  • T-cell expansion
  • Tumor antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

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  • Cite this

    Kondo, E., Gryschok, L., Schultze, J. L., & Von Bergwelt-Baildon, M. S. (2009). Using CD40-activated B cells to efficiently identify epitopes of tumor antigens. Journal of Immunotherapy, 32(2), 157-160. https://doi.org/10.1097/CJI.0b013e31819031a2