TY - JOUR
T1 - Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) to study carbon allocation in plants after herbivore attack
AU - Meldau, Stefan
AU - Woldemariam, Melkamu G.
AU - Fatangare, Amol
AU - Svatos, Ales
AU - Galis, Ivan
N1 - Funding Information:
We thank Hans-Peter Saluz, Peter Gebhardt and Thomas Opfermann for providing technical assistance with [18F]FDG and equipment at the Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany. The work of Stefan Meldau is funded by Advanced Grant No 293926 of the European Research Council to Ian Baldwin. Melkamu GW was funded by the German Academic Exchange Service (DAAD).This study is supported by the Max Planck Society.
Publisher Copyright:
© 2015 Meldau et al.; licensee BioMed Central.
PY - 2015
Y1 - 2015
N2 - Background: Although leaf herbivory-induced changes in allocation of recently assimilated carbon between the shoot and below-ground tissues have been described in several species, it is still unclear which part of the root system is affected by resource allocation changes and which signalling pathways are involved. We investigated carbon partitioning in root tissues following wounding and simulated leaf herbivory in young Nicotiana attenuata plants. Results: Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), which was incorporated into disaccharides in planta, we found that simulated herbivory reduced carbon partitioning specifically to the root tips in wild type plants. In jasmonate (JA) signalling-deficient COI1 plants, the wound-induced allocation of [18F]FDG to the roots was decreased, while more [18F]FDG was transported to young leaves, demonstrating an important role of the JA pathway in regulating the wound-induced carbon partitioning between shoots and roots. Conclusions: Our data highlight the use of [18F]FDG to study stress-induced carbon allocation responses in plants and indicate an important role of the JA pathway in regulating wound-induced shoot to root signalling.
AB - Background: Although leaf herbivory-induced changes in allocation of recently assimilated carbon between the shoot and below-ground tissues have been described in several species, it is still unclear which part of the root system is affected by resource allocation changes and which signalling pathways are involved. We investigated carbon partitioning in root tissues following wounding and simulated leaf herbivory in young Nicotiana attenuata plants. Results: Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), which was incorporated into disaccharides in planta, we found that simulated herbivory reduced carbon partitioning specifically to the root tips in wild type plants. In jasmonate (JA) signalling-deficient COI1 plants, the wound-induced allocation of [18F]FDG to the roots was decreased, while more [18F]FDG was transported to young leaves, demonstrating an important role of the JA pathway in regulating the wound-induced carbon partitioning between shoots and roots. Conclusions: Our data highlight the use of [18F]FDG to study stress-induced carbon allocation responses in plants and indicate an important role of the JA pathway in regulating wound-induced shoot to root signalling.
KW - 2-Deoxy-2-[F]fluoro-D-glucose ([F]FDG)
KW - Fatty acid-amino acid conjugates
KW - Herbivory
KW - Jasmonate signalling
KW - Nicotiana attenuata
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U2 - 10.1186/s13104-015-0989-z
DO - 10.1186/s13104-015-0989-z
M3 - Article
C2 - 25888779
AN - SCOPUS:84924157911
VL - 8
JO - BMC Research Notes
JF - BMC Research Notes
SN - 1756-0500
IS - 1
M1 - 45
ER -