Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) to study carbon allocation in plants after herbivore attack

Stefan Meldau, Melkamu G. Woldemariam, Amol Fatangare, Ales Svatos, Ivan Galis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Although leaf herbivory-induced changes in allocation of recently assimilated carbon between the shoot and below-ground tissues have been described in several species, it is still unclear which part of the root system is affected by resource allocation changes and which signalling pathways are involved. We investigated carbon partitioning in root tissues following wounding and simulated leaf herbivory in young Nicotiana attenuata plants. Results: Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), which was incorporated into disaccharides in planta, we found that simulated herbivory reduced carbon partitioning specifically to the root tips in wild type plants. In jasmonate (JA) signalling-deficient COI1 plants, the wound-induced allocation of [18F]FDG to the roots was decreased, while more [18F]FDG was transported to young leaves, demonstrating an important role of the JA pathway in regulating the wound-induced carbon partitioning between shoots and roots. Conclusions: Our data highlight the use of [18F]FDG to study stress-induced carbon allocation responses in plants and indicate an important role of the JA pathway in regulating wound-induced shoot to root signalling.

Original languageEnglish
Article number45
JournalBMC Research Notes
Volume8
Issue number1
DOIs
Publication statusPublished - 2015

Fingerprint

Herbivory
Fluorodeoxyglucose F18
Carbon
Glucose
Wounds and Injuries
Tissue
Meristem
Resource Allocation
Disaccharides
Resource allocation
Tobacco
jasmonic acid

Keywords

  • 2-Deoxy-2-[F]fluoro-D-glucose ([F]FDG)
  • Fatty acid-amino acid conjugates
  • Herbivory
  • Jasmonate signalling
  • Nicotiana attenuata

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) to study carbon allocation in plants after herbivore attack. / Meldau, Stefan; Woldemariam, Melkamu G.; Fatangare, Amol; Svatos, Ales; Galis, Ivan.

In: BMC Research Notes, Vol. 8, No. 1, 45, 2015.

Research output: Contribution to journalArticle

Meldau, Stefan ; Woldemariam, Melkamu G. ; Fatangare, Amol ; Svatos, Ales ; Galis, Ivan. / Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) to study carbon allocation in plants after herbivore attack. In: BMC Research Notes. 2015 ; Vol. 8, No. 1.
@article{3bdf95ec39744adabf5a1b55ed8bd10d,
title = "Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) to study carbon allocation in plants after herbivore attack",
abstract = "Background: Although leaf herbivory-induced changes in allocation of recently assimilated carbon between the shoot and below-ground tissues have been described in several species, it is still unclear which part of the root system is affected by resource allocation changes and which signalling pathways are involved. We investigated carbon partitioning in root tissues following wounding and simulated leaf herbivory in young Nicotiana attenuata plants. Results: Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), which was incorporated into disaccharides in planta, we found that simulated herbivory reduced carbon partitioning specifically to the root tips in wild type plants. In jasmonate (JA) signalling-deficient COI1 plants, the wound-induced allocation of [18F]FDG to the roots was decreased, while more [18F]FDG was transported to young leaves, demonstrating an important role of the JA pathway in regulating the wound-induced carbon partitioning between shoots and roots. Conclusions: Our data highlight the use of [18F]FDG to study stress-induced carbon allocation responses in plants and indicate an important role of the JA pathway in regulating wound-induced shoot to root signalling.",
keywords = "2-Deoxy-2-[F]fluoro-D-glucose ([F]FDG), Fatty acid-amino acid conjugates, Herbivory, Jasmonate signalling, Nicotiana attenuata",
author = "Stefan Meldau and Woldemariam, {Melkamu G.} and Amol Fatangare and Ales Svatos and Ivan Galis",
year = "2015",
doi = "10.1186/s13104-015-0989-z",
language = "English",
volume = "8",
journal = "BMC Research Notes",
issn = "1756-0500",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) to study carbon allocation in plants after herbivore attack

AU - Meldau, Stefan

AU - Woldemariam, Melkamu G.

AU - Fatangare, Amol

AU - Svatos, Ales

AU - Galis, Ivan

PY - 2015

Y1 - 2015

N2 - Background: Although leaf herbivory-induced changes in allocation of recently assimilated carbon between the shoot and below-ground tissues have been described in several species, it is still unclear which part of the root system is affected by resource allocation changes and which signalling pathways are involved. We investigated carbon partitioning in root tissues following wounding and simulated leaf herbivory in young Nicotiana attenuata plants. Results: Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), which was incorporated into disaccharides in planta, we found that simulated herbivory reduced carbon partitioning specifically to the root tips in wild type plants. In jasmonate (JA) signalling-deficient COI1 plants, the wound-induced allocation of [18F]FDG to the roots was decreased, while more [18F]FDG was transported to young leaves, demonstrating an important role of the JA pathway in regulating the wound-induced carbon partitioning between shoots and roots. Conclusions: Our data highlight the use of [18F]FDG to study stress-induced carbon allocation responses in plants and indicate an important role of the JA pathway in regulating wound-induced shoot to root signalling.

AB - Background: Although leaf herbivory-induced changes in allocation of recently assimilated carbon between the shoot and below-ground tissues have been described in several species, it is still unclear which part of the root system is affected by resource allocation changes and which signalling pathways are involved. We investigated carbon partitioning in root tissues following wounding and simulated leaf herbivory in young Nicotiana attenuata plants. Results: Using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG), which was incorporated into disaccharides in planta, we found that simulated herbivory reduced carbon partitioning specifically to the root tips in wild type plants. In jasmonate (JA) signalling-deficient COI1 plants, the wound-induced allocation of [18F]FDG to the roots was decreased, while more [18F]FDG was transported to young leaves, demonstrating an important role of the JA pathway in regulating the wound-induced carbon partitioning between shoots and roots. Conclusions: Our data highlight the use of [18F]FDG to study stress-induced carbon allocation responses in plants and indicate an important role of the JA pathway in regulating wound-induced shoot to root signalling.

KW - 2-Deoxy-2-[F]fluoro-D-glucose ([F]FDG)

KW - Fatty acid-amino acid conjugates

KW - Herbivory

KW - Jasmonate signalling

KW - Nicotiana attenuata

UR - http://www.scopus.com/inward/record.url?scp=84924157911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924157911&partnerID=8YFLogxK

U2 - 10.1186/s13104-015-0989-z

DO - 10.1186/s13104-015-0989-z

M3 - Article

VL - 8

JO - BMC Research Notes

JF - BMC Research Notes

SN - 1756-0500

IS - 1

M1 - 45

ER -