Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma

Yasumasa Shimoura; Yasuharu Sato; Katsuyoshi Takata; Yorihisa Orita; Satoko Nakamura; Shyouhei Mano; Tadashi Yoshino

doi:10.1159/000342475

Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma

Yasumasa Shimoura, Yasuharu Sato, Katsuyoshi Takata, Yorihisa Orita, Satoko Nakamura, Shyouhei Mano, Tadashi Yoshino

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.

Original language	English
Pages (from-to)	84-90
Number of pages	7
Journal	Acta Cytologica
Volume	57
Issue number	1
DOIs	https://doi.org/10.1159/000342475
Publication status	Published - 2013

Keywords

B cell lymphoma
Cytology
Flow cytometry
Immunocytochemical double staining
Immunoglobulin light-chain restriction
Lymph node

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology

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Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma. / Shimoura, Yasumasa; Sato, Yasuharu; Takata, Katsuyoshi; Orita, Yorihisa; Nakamura, Satoko; Mano, Shyouhei; Yoshino, Tadashi.

In: Acta Cytologica, Vol. 57, No. 1, 2013, p. 84-90.

Research output: Contribution to journal › Article › peer-review

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title = "Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma",

abstract = "Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.",

keywords = "B cell lymphoma, Cytology, Flow cytometry, Immunocytochemical double staining, Immunoglobulin light-chain restriction, Lymph node",

author = "Yasumasa Shimoura and Yasuharu Sato and Katsuyoshi Takata and Yorihisa Orita and Satoko Nakamura and Shyouhei Mano and Tadashi Yoshino",

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AU - Shimoura, Yasumasa

AU - Sato, Yasuharu

AU - Takata, Katsuyoshi

AU - Orita, Yorihisa

AU - Nakamura, Satoko

AU - Mano, Shyouhei

AU - Yoshino, Tadashi

PY - 2013

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N2 - Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.

AB - Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.

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KW - Cytology

KW - Flow cytometry

KW - Immunocytochemical double staining

KW - Immunoglobulin light-chain restriction

KW - Lymph node

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Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma

Abstract

Keywords

ASJC Scopus subject areas

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