Abstract
Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.
Original language | English |
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Pages (from-to) | 84-90 |
Number of pages | 7 |
Journal | Acta Cytologica |
Volume | 57 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- B cell lymphoma
- Cytology
- Flow cytometry
- Immunocytochemical double staining
- Immunoglobulin light-chain restriction
- Lymph node
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology