Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma

Yasumasa Shimoura, Yasuharu Sato, Katsuyoshi Takata, Yorihisa Orita, Satoko Nakamura, Shyouhei Mano, Tadashi Yoshino

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalActa Cytologica
Volume57
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • B cell lymphoma
  • Cytology
  • Flow cytometry
  • Immunocytochemical double staining
  • Immunoglobulin light-chain restriction
  • Lymph node

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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