Use of wood vinegar to enhance 5-aminolevulinic acid production by selected Rhodopseudomonas palustris in rubber sheet wastewater for agricultural use

Tomorn Nunkaew, Duangporn Kantachote, Sumate Chaiprapat, Teruhiko Nitoda, Hiroshi Kanzaki

Research output: Contribution to journalArticle

Abstract

This study aimed to produce inexpensive 5-aminolevulinic acid (ALA) in a non-sterile latex rubber sheet wastewater (RSW) by Rhodopseudomonas palustris TN114 and PP803 for the possibility to use in agricultural purposes by investigating the optimum conditions, and applying of wood vinegar (WV) as an economical source of levulinic acid to enhance ALA content. The Box-Behnken Design experiment was conducted under microaerobic-light conditions for 96. h with TN114, PP803 and their mixed culture (1:1) by varying initial pH, inoculum size (% v/v) and initial chemical oxygen demand (COD, mg/L). Results showed that the optimal condition (pH, % inoculum size, COD) of each set to produce extracellular ALA was found at 7.50, 6.00, 2000 for TN114; 7.50, 7.00, 3000 for PP803; and 7.50, 6.00, 4000 for a mixed culture; and each set achieved COD reduction as high as 63%, 71% and 75%, respectively. Addition of the optimal concentration of WV at mid log phase at 0.63% for TN114, and 1.25% for PP803 and the mixed culture significantly increased the ALA content by 3.7-4.2. times (128, 90 and 131. μM, respectively) compared to their controls. ALA production cost could be reduced approximately 31. times with WV on the basis of the amount of levulinic acid used. Effluent containing ALA for using in agriculture could be achieved by treating the RSW with the selected ALA producer R. palustris strains under the optimized condition with a little WV additive.

Original languageEnglish
JournalSaudi Journal of Biological Sciences
DOIs
Publication statusAccepted/In press - Jul 25 2015

Fingerprint

Rhodopseudomonas palustris
Rhodopseudomonas
Aminolevulinic Acid
aminolevulinic acid
vinegars
Rubber
Waste Water
rubber
wastewater
mixed culture
levulinic acid
inoculum
Biological Oxygen Demand Analysis
chemical oxygen demand
latex
wood vinegar
Agriculture
production costs
effluents
experimental design

Keywords

  • 5-Aminolevulinic acid
  • Levulinic acid
  • Response surface methodology
  • Rhodopseudomonas spp.
  • Rubber wastewater
  • Wood vinegar

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Use of wood vinegar to enhance 5-aminolevulinic acid production by selected Rhodopseudomonas palustris in rubber sheet wastewater for agricultural use. / Nunkaew, Tomorn; Kantachote, Duangporn; Chaiprapat, Sumate; Nitoda, Teruhiko; Kanzaki, Hiroshi.

In: Saudi Journal of Biological Sciences, 25.07.2015.

Research output: Contribution to journalArticle

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abstract = "This study aimed to produce inexpensive 5-aminolevulinic acid (ALA) in a non-sterile latex rubber sheet wastewater (RSW) by Rhodopseudomonas palustris TN114 and PP803 for the possibility to use in agricultural purposes by investigating the optimum conditions, and applying of wood vinegar (WV) as an economical source of levulinic acid to enhance ALA content. The Box-Behnken Design experiment was conducted under microaerobic-light conditions for 96. h with TN114, PP803 and their mixed culture (1:1) by varying initial pH, inoculum size ({\%} v/v) and initial chemical oxygen demand (COD, mg/L). Results showed that the optimal condition (pH, {\%} inoculum size, COD) of each set to produce extracellular ALA was found at 7.50, 6.00, 2000 for TN114; 7.50, 7.00, 3000 for PP803; and 7.50, 6.00, 4000 for a mixed culture; and each set achieved COD reduction as high as 63{\%}, 71{\%} and 75{\%}, respectively. Addition of the optimal concentration of WV at mid log phase at 0.63{\%} for TN114, and 1.25{\%} for PP803 and the mixed culture significantly increased the ALA content by 3.7-4.2. times (128, 90 and 131. μM, respectively) compared to their controls. ALA production cost could be reduced approximately 31. times with WV on the basis of the amount of levulinic acid used. Effluent containing ALA for using in agriculture could be achieved by treating the RSW with the selected ALA producer R. palustris strains under the optimized condition with a little WV additive.",
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