Use of silkworm larvae to study pathogenic bacterial toxins

Muktadir S. Hossain, Hiroshi Hamamoto, Yasuhiko Matsumoto, Iony M. Razanajatovo, Jorge Larranaga, Chikara Kaito, Hiroshi Kasuga, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Injection of stationary phase culture-supernatants of Staphylococcus aureus and Pseudomonas aeruginosa into the hemolymph of silkworm larvae caused their death, whereas a culture-supernatant of a non-pathogenic strain of Escherichia coli did not. A culture-supernatant of a mutant of agr, a global virulence regulator of S. aureus that is required for exotoxin production, was much less toxic to silkworm larvae. A culture-supernatant of a disruption mutant of the S. aureus beta-toxin gene did not kill larvae, whereas one of a deletion mutant of alpha-toxin, gamma-toxin, or aureolysin killed larvae, indicating that the beta-toxin gene is required for staphylococcal supernatant-mediated killing of silkworm larvae. The 50% lethal doses (LD50) of staphylococcal alpha-toxin and beta-toxin, Pseudomonas exotoxin A and diphtheria toxin were 12 μg/g, 9 μg/g, 0.14 μg/g and 1.1 μg/g, respectively. As the purified toxins killed the larvae, silkworm larvae could be used as a model to study the actions of pathogenic bacterial toxins in animal bodies.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalJournal of biochemistry
Volume140
Issue number3
DOIs
Publication statusPublished - Sep 1 2006
Externally publishedYes

Fingerprint

Bacterial Toxins
Bombyx
Larva
Genes
Diphtheria Toxin
Exotoxins
Poisons
Escherichia coli
Animals
Lethal Dose 50
Staphylococcus aureus
Hemolymph
Pseudomonas aeruginosa
Virulence
Injections
aureolysin
Pseudomonas aeruginosa toxA protein
staphylococcal alpha-toxin
Staphylococcus aureus hlb protein

Keywords

  • Animal model
  • Infection
  • Pathogenic bacterial toxins
  • Silkworm larva
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Hossain, M. S., Hamamoto, H., Matsumoto, Y., Razanajatovo, I. M., Larranaga, J., Kaito, C., ... Sekimizu, K. (2006). Use of silkworm larvae to study pathogenic bacterial toxins. Journal of biochemistry, 140(3), 439-444. https://doi.org/10.1093/jb/mvj171

Use of silkworm larvae to study pathogenic bacterial toxins. / Hossain, Muktadir S.; Hamamoto, Hiroshi; Matsumoto, Yasuhiko; Razanajatovo, Iony M.; Larranaga, Jorge; Kaito, Chikara; Kasuga, Hiroshi; Sekimizu, Kazuhisa.

In: Journal of biochemistry, Vol. 140, No. 3, 01.09.2006, p. 439-444.

Research output: Contribution to journalArticle

Hossain, MS, Hamamoto, H, Matsumoto, Y, Razanajatovo, IM, Larranaga, J, Kaito, C, Kasuga, H & Sekimizu, K 2006, 'Use of silkworm larvae to study pathogenic bacterial toxins', Journal of biochemistry, vol. 140, no. 3, pp. 439-444. https://doi.org/10.1093/jb/mvj171
Hossain MS, Hamamoto H, Matsumoto Y, Razanajatovo IM, Larranaga J, Kaito C et al. Use of silkworm larvae to study pathogenic bacterial toxins. Journal of biochemistry. 2006 Sep 1;140(3):439-444. https://doi.org/10.1093/jb/mvj171
Hossain, Muktadir S. ; Hamamoto, Hiroshi ; Matsumoto, Yasuhiko ; Razanajatovo, Iony M. ; Larranaga, Jorge ; Kaito, Chikara ; Kasuga, Hiroshi ; Sekimizu, Kazuhisa. / Use of silkworm larvae to study pathogenic bacterial toxins. In: Journal of biochemistry. 2006 ; Vol. 140, No. 3. pp. 439-444.
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