Use of liposomes to study vesicular transport.

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.

Original languageEnglish
Pages (from-to)531-542
Number of pages12
JournalMethods in Molecular Biology
Volume606
Publication statusPublished - 2010

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Clathrin
Liposomes
Unilamellar Liposomes
Styrene
Cell-Free System
Capsid Proteins
Endocytosis
Phagocytosis
Membranes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Use of liposomes to study vesicular transport. / Takei, Kohji; Yamada, Hiroshi; Abe, Tadashi.

In: Methods in Molecular Biology, Vol. 606, 2010, p. 531-542.

Research output: Contribution to journalArticle

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