Use of liposomes to study vesicular transport.

Kohji Takei; Hiroshi Yamada; Tadashi Abe

doi:10.1007/978-1-60761-447-0_36

Use of liposomes to study vesicular transport.

Kohji Takei, Hiroshi Yamada, Tadashi Abe

Research output: Contribution to journal › Article › peer-review

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Abstract

Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.

Original language	English
Pages (from-to)	531-542
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	606
DOIs	https://doi.org/10.1007/978-1-60761-447-0_36
Publication status	Published - 2010

ASJC Scopus subject areas

Molecular Biology
Genetics

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AB - Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.

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Use of liposomes to study vesicular transport.

Abstract

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