Abstract
Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
Original language | English |
---|---|
Pages (from-to) | 531-542 |
Number of pages | 12 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 606 |
DOIs | |
Publication status | Published - 2010 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics