Use of liposomes to study vesicular transport.

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Abstract

Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.

Original languageEnglish
Pages (from-to)531-542
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume606
Publication statusPublished - 2010

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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