Abstract
Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 531-542 |
| Number of pages | 12 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 606 |
| Publication status | Published - 2010 |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Takei, K., Yamada, H., & Abe, T. (2010). Use of liposomes to study vesicular transport. Methods in molecular biology (Clifton, N.J.), 606, 531-542.