Abstract
Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 531-542 |
| Number of pages | 12 |
| Journal | Methods in Molecular Biology |
| Volume | 606 |
| Publication status | Published - 2010 |
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- Medicine(all)
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Use of liposomes to study vesicular transport. / Takei, Kohji; Yamada, Hiroshi; Abe, Tadashi.
In: Methods in Molecular Biology, Vol. 606, 2010, p. 531-542.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of liposomes to study vesicular transport.
AU - Takei, Kohji
AU - Yamada, Hiroshi
AU - Abe, Tadashi
PY - 2010
Y1 - 2010
N2 - Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
AB - Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
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UR - http://www.scopus.com/inward/citedby.url?scp=77649195553&partnerID=8YFLogxK
M3 - Article
C2 - 20013420
AN - SCOPUS:77649195553
VL - 606
SP - 531
EP - 542
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -