Use of lipid disperse systems in transdermal drug delivery: Comparative study of flufenamic acid permeation among rat abdominal skin, silicon rubber membrane and stratum corneum sheet isolated from hamster cheek pouch

Yuji Kurosaki, Naoki Nagahara, Toshihiro Tanizawa, Hidekatsu Nishimura, Taiji Nakayama, Toshikiro Kimura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The characteristics of in vitro permeation of flufenamic acid (FA) from lipid disperse systems composed of phosphatidylcholine (PC) and glycosylceramide (GC) were compared among rat abdominal skin, a silicon rubber membrane (Silastic®) and a stratum corneum (SC) sheet isolated from hamster cheek pouch. When a PC dispersion (PCD) containing 20 μmol PC/ml was applied, the permeation of FA through rat skin was enhanced approx. 2.2-fold compared with that from the lipid-free suspension (LFS). Further, a nearly 2-fold enhancement was observed when a GC-containing PCD (10% GC-PCD) was examined. A similar pattern of enhancement could be reproduced when cheek pouch SC was used instead of, rat skin, whereas it was not observed in Silastic®. The enhanced permeation in the skin could not be explained on the basis of the incremental increase in the apparent solubilities. A significant correlation was observed between skin permeation and epidermal tissue uptake of FA from LFS and PCDs, although the nearly 2-fold increase found in 10% GC-PCD might be due to mechanisms other than the increase in epidermal tissue uptake. The usefulness of an SC sheet isolated from hamster cheek pouch, a new model membrane without appendages, in studying the direct action of either permeation enhancers or dosage forms designed to enhance the percutaneous permeation of drugs on the SC is discussed.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume67
Issue number1
DOIs
Publication statusPublished - Jan 1 1991

Fingerprint

Flufenamic Acid
Cheek
Rubber
Silicon
Cricetinae
Cornea
Lipids
Skin
Phosphatidylcholines
Membranes
Pharmaceutical Preparations
Suspensions
Dosage Forms
Solubility

Keywords

  • Flufenamic acid
  • Hamster cheek pouch
  • Isolated stratum corneum
  • Lipid disperse system
  • Percutaneous permeation
  • Rat abdominal skin
  • Silastic

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Use of lipid disperse systems in transdermal drug delivery : Comparative study of flufenamic acid permeation among rat abdominal skin, silicon rubber membrane and stratum corneum sheet isolated from hamster cheek pouch. / Kurosaki, Yuji; Nagahara, Naoki; Tanizawa, Toshihiro; Nishimura, Hidekatsu; Nakayama, Taiji; Kimura, Toshikiro.

In: International Journal of Pharmaceutics, Vol. 67, No. 1, 01.01.1991, p. 1-9.

Research output: Contribution to journalArticle

@article{3760c082358b49b8848955479ac08322,
title = "Use of lipid disperse systems in transdermal drug delivery: Comparative study of flufenamic acid permeation among rat abdominal skin, silicon rubber membrane and stratum corneum sheet isolated from hamster cheek pouch",
abstract = "The characteristics of in vitro permeation of flufenamic acid (FA) from lipid disperse systems composed of phosphatidylcholine (PC) and glycosylceramide (GC) were compared among rat abdominal skin, a silicon rubber membrane (Silastic{\circledR}) and a stratum corneum (SC) sheet isolated from hamster cheek pouch. When a PC dispersion (PCD) containing 20 μmol PC/ml was applied, the permeation of FA through rat skin was enhanced approx. 2.2-fold compared with that from the lipid-free suspension (LFS). Further, a nearly 2-fold enhancement was observed when a GC-containing PCD (10{\%} GC-PCD) was examined. A similar pattern of enhancement could be reproduced when cheek pouch SC was used instead of, rat skin, whereas it was not observed in Silastic{\circledR}. The enhanced permeation in the skin could not be explained on the basis of the incremental increase in the apparent solubilities. A significant correlation was observed between skin permeation and epidermal tissue uptake of FA from LFS and PCDs, although the nearly 2-fold increase found in 10{\%} GC-PCD might be due to mechanisms other than the increase in epidermal tissue uptake. The usefulness of an SC sheet isolated from hamster cheek pouch, a new model membrane without appendages, in studying the direct action of either permeation enhancers or dosage forms designed to enhance the percutaneous permeation of drugs on the SC is discussed.",
keywords = "Flufenamic acid, Hamster cheek pouch, Isolated stratum corneum, Lipid disperse system, Percutaneous permeation, Rat abdominal skin, Silastic",
author = "Yuji Kurosaki and Naoki Nagahara and Toshihiro Tanizawa and Hidekatsu Nishimura and Taiji Nakayama and Toshikiro Kimura",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0378-5173(91)90259-Q",
language = "English",
volume = "67",
pages = "1--9",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Use of lipid disperse systems in transdermal drug delivery

T2 - Comparative study of flufenamic acid permeation among rat abdominal skin, silicon rubber membrane and stratum corneum sheet isolated from hamster cheek pouch

AU - Kurosaki, Yuji

AU - Nagahara, Naoki

AU - Tanizawa, Toshihiro

AU - Nishimura, Hidekatsu

AU - Nakayama, Taiji

AU - Kimura, Toshikiro

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The characteristics of in vitro permeation of flufenamic acid (FA) from lipid disperse systems composed of phosphatidylcholine (PC) and glycosylceramide (GC) were compared among rat abdominal skin, a silicon rubber membrane (Silastic®) and a stratum corneum (SC) sheet isolated from hamster cheek pouch. When a PC dispersion (PCD) containing 20 μmol PC/ml was applied, the permeation of FA through rat skin was enhanced approx. 2.2-fold compared with that from the lipid-free suspension (LFS). Further, a nearly 2-fold enhancement was observed when a GC-containing PCD (10% GC-PCD) was examined. A similar pattern of enhancement could be reproduced when cheek pouch SC was used instead of, rat skin, whereas it was not observed in Silastic®. The enhanced permeation in the skin could not be explained on the basis of the incremental increase in the apparent solubilities. A significant correlation was observed between skin permeation and epidermal tissue uptake of FA from LFS and PCDs, although the nearly 2-fold increase found in 10% GC-PCD might be due to mechanisms other than the increase in epidermal tissue uptake. The usefulness of an SC sheet isolated from hamster cheek pouch, a new model membrane without appendages, in studying the direct action of either permeation enhancers or dosage forms designed to enhance the percutaneous permeation of drugs on the SC is discussed.

AB - The characteristics of in vitro permeation of flufenamic acid (FA) from lipid disperse systems composed of phosphatidylcholine (PC) and glycosylceramide (GC) were compared among rat abdominal skin, a silicon rubber membrane (Silastic®) and a stratum corneum (SC) sheet isolated from hamster cheek pouch. When a PC dispersion (PCD) containing 20 μmol PC/ml was applied, the permeation of FA through rat skin was enhanced approx. 2.2-fold compared with that from the lipid-free suspension (LFS). Further, a nearly 2-fold enhancement was observed when a GC-containing PCD (10% GC-PCD) was examined. A similar pattern of enhancement could be reproduced when cheek pouch SC was used instead of, rat skin, whereas it was not observed in Silastic®. The enhanced permeation in the skin could not be explained on the basis of the incremental increase in the apparent solubilities. A significant correlation was observed between skin permeation and epidermal tissue uptake of FA from LFS and PCDs, although the nearly 2-fold increase found in 10% GC-PCD might be due to mechanisms other than the increase in epidermal tissue uptake. The usefulness of an SC sheet isolated from hamster cheek pouch, a new model membrane without appendages, in studying the direct action of either permeation enhancers or dosage forms designed to enhance the percutaneous permeation of drugs on the SC is discussed.

KW - Flufenamic acid

KW - Hamster cheek pouch

KW - Isolated stratum corneum

KW - Lipid disperse system

KW - Percutaneous permeation

KW - Rat abdominal skin

KW - Silastic

UR - http://www.scopus.com/inward/record.url?scp=0026068604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026068604&partnerID=8YFLogxK

U2 - 10.1016/0378-5173(91)90259-Q

DO - 10.1016/0378-5173(91)90259-Q

M3 - Article

AN - SCOPUS:0026068604

VL - 67

SP - 1

EP - 9

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1

ER -