Use of DNA-generated gold nanoparticles to radiosensitize and eradicate radioresistant glioma stem cells

Tatsuki Kunoh, Tsutomu Shimura, Tomonari Kasai, Syuji Matsumoto, Hafizah Mahmud, Apriliana Cahya Khayrani, Masaharu Seno, Hitoshi Kunoh, Jun Takada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The surface reactivity of gold nanoparticles (AuNPs) is receiving attention as a radiosensitizer of cancer cells for radiation therapy and/or as a drug carrier to target cells. This study demonstrates the potential of DNA-AuNPs (prepared by mixing calf thymus DNA with HAuCl4 solution) as a radiosensitizer of human glioma cells that have cancer stem cell (CSC)-like properties, to reduce their survival. CSC-like U251MG-P1 cells and their parental glioblastoma U251MG cells are treated with a prepared DNA-AuNP colloid. The radiosensitivity of the resultant AuNP-associated cells are significantly enhanced. To reveal the mechanism by which survival is reduced, the generation of reactive oxygen species (ROS), apoptosis induction, or DNA damage in the cells is assayed using the fluorescent dye DCFDA, annexin V-FITC/PI, and foci formation of γ-H2AX, respectively. X-ray irradiation with administration of AuNPs overcomes the radioresistance of U251MG-P1 cells. It does not induce ROS generation or apoptosis in the cells but enhances the number of abnormal nuclei with abundant γ-H2AX foci, which is judged as cell death by mitotic catastrophe. The AuNP association with the cells effectively induces mitotic catastrophe in x-ray-irradiated CSC-like cells, implicating that DNA-AuNPs might be a promising tool to develop an efficient radiosensitizer against CSC.

Original languageEnglish
Article number055101
JournalNanotechnology
Volume30
Issue number5
DOIs
Publication statusPublished - Feb 1 2019

Fingerprint

Stem cells
Gold
DNA
Nanoparticles
Cell death
Reactive Oxygen Species
Apoptosis
X rays
Drug Carriers
Fluorescein-5-isothiocyanate
Annexin A5
Radiotherapy
Colloids
Thymus
Fluorescent Dyes
Oxygen
Cells
Irradiation
Dyes

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Kunoh, T., Shimura, T., Kasai, T., Matsumoto, S., Mahmud, H., Khayrani, A. C., ... Takada, J. (2019). Use of DNA-generated gold nanoparticles to radiosensitize and eradicate radioresistant glioma stem cells. Nanotechnology, 30(5), [055101]. https://doi.org/10.1088/1361-6528/aaedd5

Use of DNA-generated gold nanoparticles to radiosensitize and eradicate radioresistant glioma stem cells. / Kunoh, Tatsuki; Shimura, Tsutomu; Kasai, Tomonari; Matsumoto, Syuji; Mahmud, Hafizah; Khayrani, Apriliana Cahya; Seno, Masaharu; Kunoh, Hitoshi; Takada, Jun.

In: Nanotechnology, Vol. 30, No. 5, 055101, 01.02.2019.

Research output: Contribution to journalArticle

Kunoh, T, Shimura, T, Kasai, T, Matsumoto, S, Mahmud, H, Khayrani, AC, Seno, M, Kunoh, H & Takada, J 2019, 'Use of DNA-generated gold nanoparticles to radiosensitize and eradicate radioresistant glioma stem cells', Nanotechnology, vol. 30, no. 5, 055101. https://doi.org/10.1088/1361-6528/aaedd5
Kunoh, Tatsuki ; Shimura, Tsutomu ; Kasai, Tomonari ; Matsumoto, Syuji ; Mahmud, Hafizah ; Khayrani, Apriliana Cahya ; Seno, Masaharu ; Kunoh, Hitoshi ; Takada, Jun. / Use of DNA-generated gold nanoparticles to radiosensitize and eradicate radioresistant glioma stem cells. In: Nanotechnology. 2019 ; Vol. 30, No. 5.
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