Use of αv Integrin Linked to GFP to Image Molecular Dynamics in Trafficking Cancer-Cell Emboli

Yasunori Tome, Shuya Yano, Naotoshi Sugimoto, Sumiyuki Mii, Michael Bouvet, Hiroyuki Tsuchiya, Fuminori Kanaya, Robert M. Hoffman

Research output: Contribution to journalArticle

Abstract

Human osteosarcoma cells with an αv integrin green fluorescent protein (GFP) fusion gene were previously established and imaged in vitro and in vivo. In the present study, we imaged the interaction of αv integrin-GFP in osteosarcoma cells and collagen fibers in vascular-trafficking osteosarcoma emboli in nude mice. Human 143B osteosarcoma cells, expressing αv integrin-GFP, were injected by a vascular route in an abdominal skin flap in nude mice. Osteosarcoma cells were fluorescently imaged in the epigastric cranialis vein in the abdominal skin flap by confocal microscopy. Collagen fibers were imaged in reflectance mode. At early stages of tumor embolus-formation, cancer cells adhered firmly to each other, diffusely expressing αv integrin-GFP. Two weeks after injection, collagen fiber scaffolds were visualized at the margins of tumor emboli or within them. Four weeks after injection, cancer cells invading from emboli were strongly expressing αv integrin-GFP, and were aligned along collagen fibers. The results suggest αv integrin and collagen fiber scaffolds are important for tumor embolus formation, which are potential seeds of metastasis. J. Cell. Biochem. 118: 26–30, 2017.

Original languageEnglish
Pages (from-to)26-30
Number of pages5
JournalJournal of Cellular Biochemistry
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • COLLAGEN FIBER SCAFFOLD
  • CONFOCAL MICROSCOPY
  • CONFOCAL MICROSCOPY
  • GREEN FLUORESCENT PROTEIN
  • OSTEOSARCOMA
  • REAL-TIME IMAGING
  • α INTEGRIN

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Use of α<sub>v</sub> Integrin Linked to GFP to Image Molecular Dynamics in Trafficking Cancer-Cell Emboli'. Together they form a unique fingerprint.

  • Cite this

    Tome, Y., Yano, S., Sugimoto, N., Mii, S., Bouvet, M., Tsuchiya, H., Kanaya, F., & Hoffman, R. M. (2017). Use of αv Integrin Linked to GFP to Image Molecular Dynamics in Trafficking Cancer-Cell Emboli. Journal of Cellular Biochemistry, 118(1), 26-30. https://doi.org/10.1002/jcb.25603