Uptake of [3H]L-serine in rat brain synaptosomal fractions

Takeshi Takarada, Vladimir J. Balcar, Katsuhiro Baba, Akiko Takamoto, Gabriela B. Acosta, Katsura Takano, Yukio Yoneda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Accumulation of [3H]L-serine in crude synaptosomal fractions freshly prepared from rat brain has been found to be temperature-sensitive and to consist of both Na+-dependent and Na+-independent components. The accumulation of [3H]L-serine measured at submicromolar concentrations had a distinct substrate selectivity, different from the uptake of [3H]L-proline, [3H]L-glutamate and [3H]GABA. It was fully inhibited by L-glutamine, L-asparagine, L-cysteine, L-alanine, L-leucine, L-isoleucine, L-tyrosine, L-phenylalanine, L-threonine and by the synthetic marker for the large neutral amino acid transport systems 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid, but not influenced by β-alanine, taurine, glycine nor was it inhibited by the marker for the A system, L-2-methylamino isobutyric acid. D-Serine at 1 mM concentration produced no significant inhibition of the accumulation of 10 nM [3H]L-serine. We conclude that L-serine uptake observed in the present study is mediated by at least two distinct transport systems: a Na+-dependent one of lower affinity (Km in mM range) and a Na+-independent system of higher affinity (Km∼20-100 μM). Characteristics of [3H]L-serine accumulation displayed at low substrate concentrations suggest that it was mediated neither by the typical 'A', nor by the 'large neutral', amino acid transport systems but predominantly by transporters belonging to the recently identified LAT (L-amino acid transporter) family.

Original languageEnglish
Pages (from-to)36-47
Number of pages12
JournalBrain Research
Volume983
Issue number1-2
DOIs
Publication statusPublished - Sep 5 2003
Externally publishedYes

Keywords

  • LAT
  • Large neutral amino acid
  • Small neutral amino acid
  • System A
  • System L
  • Transport

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Uptake of [<sup>3</sup>H]L-serine in rat brain synaptosomal fractions'. Together they form a unique fingerprint.

  • Cite this

    Takarada, T., Balcar, V. J., Baba, K., Takamoto, A., Acosta, G. B., Takano, K., & Yoneda, Y. (2003). Uptake of [3H]L-serine in rat brain synaptosomal fractions. Brain Research, 983(1-2), 36-47. https://doi.org/10.1016/S0006-8993(03)03024-5