Uptake of histamine by mouse peritoneal macrophages and a macrophage cell line, RAW264.7

Satoshi Tanaka, Katsuya Deai, Mariko Inagaki, Atsushi Ichikawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have previously demonstrated that dietary histamine is accumulated in the spleens of L-histidine decarboxylase (HDC)-deficient mice, which lack endogenous histamine synthesis. To characterize the clearance system for dietary histamine in mice, we investigated the cell type and mechanism responsible for histamine uptake in the spleens of HDC-deficient mice. Immunohistochemical analyses using an antihistamine antibody indicated that a portion of the CD14+ cells in the spleen is involved in histamine storage. Peritoneal macrophages obtained from Balb/c mice and a mouse macrophage cell line, RAW264.7, had potential for histamine uptake, which was characterized by a low affinity and high capacity for histamine. The histamine uptake by RAW264.7 cells was observed at physiological temperature and was potently inhibited by pyrilamine, chlorpromazine, quinidine, and chloroquine, moderately inhibited by Nα-methylhistamine, dopamine, and serotonin, and not affected by tetraethylammonium and 1-methyl-4-phenylpyridinium. Intracellular histamine was not metabolized in RAW264.7 cells and was released at physiological temperature in the absence of extracellular histamine. These results suggest that histamine uptake by macrophages may be involved in the clearance of histamine in the local histamine-enriched environment.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume285
Issue number3 54-3
Publication statusPublished - Sep 1 2003
Externally publishedYes

Fingerprint

Macrophages
Peritoneal Macrophages
Histamine
Cells
Cell Line
Histidine Decarboxylase
Spleen
Histidine
1-Methyl-4-phenylpyridinium
Pyrilamine
Temperature
Tetraethylammonium
Quinidine
Histamine Antagonists
Chlorpromazine
Chloroquine
Dopamine
Serotonin

Keywords

  • Cation transporter
  • Chlorpromazine
  • Pyrilamine
  • Quinidine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Uptake of histamine by mouse peritoneal macrophages and a macrophage cell line, RAW264.7. / Tanaka, Satoshi; Deai, Katsuya; Inagaki, Mariko; Ichikawa, Atsushi.

In: American Journal of Physiology - Cell Physiology, Vol. 285, No. 3 54-3, 01.09.2003.

Research output: Contribution to journalArticle

Tanaka, Satoshi ; Deai, Katsuya ; Inagaki, Mariko ; Ichikawa, Atsushi. / Uptake of histamine by mouse peritoneal macrophages and a macrophage cell line, RAW264.7. In: American Journal of Physiology - Cell Physiology. 2003 ; Vol. 285, No. 3 54-3.
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