Abstract
The in vivo uptake by hepatocytes and biliary excretion of fluorescein isothiocyanate-labeled polystyrene microsphere with a particle size of 50 nm (MS-50) after intravenous administration was studied in rats. It was confirmed by using confocal laser scanning microscopy that MS-50 was partially phagocytosed by the hepatocytes and that MS-50 taken up by the hepatocytes existed exclusively inside the cells 1 h after intravenous administration. Studies on the mechanism of the uptake of MS-50 by the hepatocytes using the liver perfusion technique revealed that a process mediated by apo-E was involved. After intravenous administration of MS-50, about 4% of dose was excreted into bile in 24 h. Pharmacokinetic evaluation of the excretion rate of MS-50 into bile showed that the process followed first-order kinetics. Qualitative evaluation of the fluorescence detected in the bile after intravenous administration of MS-50 revealed that the particles were certainly excreted into bile in an intact form. From these results, it was suggested that intravenously administered MS-50 would be partially phagocytosed by hepatocytes through a process mediated by apo-E and that MS-50 ingested by hepatocytes would be partially excreted into the bile.
| Original language | English |
|---|---|
| Pages (from-to) | 213-221 |
| Number of pages | 9 |
| Journal | Journal of Drug Targeting |
| Volume | 7 |
| Issue number | 3 |
| Publication status | Published - 1999 |
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Keywords
- Biliary excretion
- Hepatocytes
- Opsonin
- Phagocytosis
- Polystyrene microsphere
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microspheres in rats. / Ogawara, Ken Ichi; Yoshida, Minoru; Furumoto, Kentaro; Takakura, Yoshinobu; Hashida, Mitsuru; Higaki, Kazutaka; Kimura, Toshikiro.
In: Journal of Drug Targeting, Vol. 7, No. 3, 1999, p. 213-221.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microspheres in rats
AU - Ogawara, Ken Ichi
AU - Yoshida, Minoru
AU - Furumoto, Kentaro
AU - Takakura, Yoshinobu
AU - Hashida, Mitsuru
AU - Higaki, Kazutaka
AU - Kimura, Toshikiro
PY - 1999
Y1 - 1999
N2 - The in vivo uptake by hepatocytes and biliary excretion of fluorescein isothiocyanate-labeled polystyrene microsphere with a particle size of 50 nm (MS-50) after intravenous administration was studied in rats. It was confirmed by using confocal laser scanning microscopy that MS-50 was partially phagocytosed by the hepatocytes and that MS-50 taken up by the hepatocytes existed exclusively inside the cells 1 h after intravenous administration. Studies on the mechanism of the uptake of MS-50 by the hepatocytes using the liver perfusion technique revealed that a process mediated by apo-E was involved. After intravenous administration of MS-50, about 4% of dose was excreted into bile in 24 h. Pharmacokinetic evaluation of the excretion rate of MS-50 into bile showed that the process followed first-order kinetics. Qualitative evaluation of the fluorescence detected in the bile after intravenous administration of MS-50 revealed that the particles were certainly excreted into bile in an intact form. From these results, it was suggested that intravenously administered MS-50 would be partially phagocytosed by hepatocytes through a process mediated by apo-E and that MS-50 ingested by hepatocytes would be partially excreted into the bile.
AB - The in vivo uptake by hepatocytes and biliary excretion of fluorescein isothiocyanate-labeled polystyrene microsphere with a particle size of 50 nm (MS-50) after intravenous administration was studied in rats. It was confirmed by using confocal laser scanning microscopy that MS-50 was partially phagocytosed by the hepatocytes and that MS-50 taken up by the hepatocytes existed exclusively inside the cells 1 h after intravenous administration. Studies on the mechanism of the uptake of MS-50 by the hepatocytes using the liver perfusion technique revealed that a process mediated by apo-E was involved. After intravenous administration of MS-50, about 4% of dose was excreted into bile in 24 h. Pharmacokinetic evaluation of the excretion rate of MS-50 into bile showed that the process followed first-order kinetics. Qualitative evaluation of the fluorescence detected in the bile after intravenous administration of MS-50 revealed that the particles were certainly excreted into bile in an intact form. From these results, it was suggested that intravenously administered MS-50 would be partially phagocytosed by hepatocytes through a process mediated by apo-E and that MS-50 ingested by hepatocytes would be partially excreted into the bile.
KW - Biliary excretion
KW - Hepatocytes
KW - Opsonin
KW - Phagocytosis
KW - Polystyrene microsphere
UR - http://www.scopus.com/inward/record.url?scp=0033378774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033378774&partnerID=8YFLogxK
M3 - Article
C2 - 10680977
AN - SCOPUS:0033378774
VL - 7
SP - 213
EP - 221
JO - Journal of Drug Targeting
JF - Journal of Drug Targeting
SN - 1061-186X
IS - 3
ER -