Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microspheres in rats

Ken Ichi Ogawara, Minoru Yoshida, Kentaro Furumoto, Yoshinobu Takakura, Mitsuru Hashida, Kazutaka Higaki, Toshikiro Kimura

Research output: Contribution to journalArticle

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Abstract

The in vivo uptake by hepatocytes and biliary excretion of fluorescein isothiocyanate-labeled polystyrene microsphere with a particle size of 50 nm (MS-50) after intravenous administration was studied in rats. It was confirmed by using confocal laser scanning microscopy that MS-50 was partially phagocytosed by the hepatocytes and that MS-50 taken up by the hepatocytes existed exclusively inside the cells 1 h after intravenous administration. Studies on the mechanism of the uptake of MS-50 by the hepatocytes using the liver perfusion technique revealed that a process mediated by apo-E was involved. After intravenous administration of MS-50, about 4% of dose was excreted into bile in 24 h. Pharmacokinetic evaluation of the excretion rate of MS-50 into bile showed that the process followed first-order kinetics. Qualitative evaluation of the fluorescence detected in the bile after intravenous administration of MS-50 revealed that the particles were certainly excreted into bile in an intact form. From these results, it was suggested that intravenously administered MS-50 would be partially phagocytosed by hepatocytes through a process mediated by apo-E and that MS-50 ingested by hepatocytes would be partially excreted into the bile.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalJournal of Drug Targeting
Volume7
Issue number3
Publication statusPublished - 1999

Fingerprint

Polystyrenes
Microspheres
Hepatocytes
Bile
Intravenous Administration
Apolipoproteins E
Phagocytosis
Fluorescein
Particle Size
Confocal Microscopy
Hepatobiliary Elimination
Pharmacokinetics
Perfusion
Fluorescence
Liver

Keywords

  • Biliary excretion
  • Hepatocytes
  • Opsonin
  • Phagocytosis
  • Polystyrene microsphere

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Ogawara, K. I., Yoshida, M., Furumoto, K., Takakura, Y., Hashida, M., Higaki, K., & Kimura, T. (1999). Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microspheres in rats. Journal of Drug Targeting, 7(3), 213-221.

Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microspheres in rats. / Ogawara, Ken Ichi; Yoshida, Minoru; Furumoto, Kentaro; Takakura, Yoshinobu; Hashida, Mitsuru; Higaki, Kazutaka; Kimura, Toshikiro.

In: Journal of Drug Targeting, Vol. 7, No. 3, 1999, p. 213-221.

Research output: Contribution to journalArticle

Ogawara, KI, Yoshida, M, Furumoto, K, Takakura, Y, Hashida, M, Higaki, K & Kimura, T 1999, 'Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microspheres in rats', Journal of Drug Targeting, vol. 7, no. 3, pp. 213-221.
Ogawara, Ken Ichi ; Yoshida, Minoru ; Furumoto, Kentaro ; Takakura, Yoshinobu ; Hashida, Mitsuru ; Higaki, Kazutaka ; Kimura, Toshikiro. / Uptake by hepatocytes and biliary excretion of intravenously administered polystyrene microspheres in rats. In: Journal of Drug Targeting. 1999 ; Vol. 7, No. 3. pp. 213-221.
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