Upsurge and spread of G3 rotaviruses in Eastern India (2014–2016): Full genome analyses reveals heterogeneity within Wa-like genomic constellation

Anindita Banerjee, Mahadeb Lo, Pallavi Indwar, Alok K. Deb, Santasabuj Das, Byomkesh Manna, Shanta Dutta, Uchhal K. Bhadra, Mala Bhattacharya, Keinosuke Okamoto, Mamta Chawla-Sarkar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Advent of new strains and shift in predominantly circulating genotypes are characteristics of group- A rotavirus (RVA), one of the major causes of childhood gastroenteritis. During diarrheal disease surveillance at Kolkata, India (2014–2016), a shift in circulating RVA strains from G1P[8] to G3P[8] was seen. Stool samples from children (n = 3048) with acute gastroenteritis were tested of which 38.7% were RVA positive. G1 was the predominant strain (65.3%) in 2014–2015 whereas in late 2015 and 2016, G3 became the preponderant strain (44.6%). In the past decade G3 strains were not observed in this region, we conducted whole genome sequencing of representative strains to gain insight into the phenomenon of emergence and genetic constellation of these circulating human G3 strains. The analyses revealed intergenogroup reassortment in G3P[4] strains (among Wa and DS-1-like genogroup) whereas G3P[8] strains were authentic Wa-like. Phylogenetic analysis revealed Kolkata G3 strains as polymorphic and thus they formed two sub-clusters due to antigenic differences in their VP7 protein. One of the sub-clusters had the wild-type threonine at 87 amino acid position while another sub-cluster had an isoleucine mutation. Presence of additional N-linked glycosylation site at amino acid 283 of VP7 glycoprotein suggests that the major neutralizing epitope on the VP7 (G3) of RotaTeq vaccine differs from the currently circulating G3 strains. The study is important as efficiency of rotavirus vaccine depends on the circulating heterogeneous genotype constellations. Continuous monitoring of circulating RVA strains in endemic settings like India is therefore important in pre- and post-vaccination period to monitor the emergence of new reassortant genotypes in addition to assessing vaccine efficacy.

Original languageEnglish
Pages (from-to)158-174
Number of pages17
JournalInfection, Genetics and Evolution
Volume63
DOIs
Publication statusPublished - Sep 1 2018
Externally publishedYes

Fingerprint

Rotavirus
vaccine
gastroenteritis
India
genomics
genotype
genome
vaccination
Genotype
Genome
Gastroenteritis
amino acid
diarrheal disease
Vaccination
Vaccines
Genetic Phenomena
Rotavirus Vaccines
Amino Acids
Isoleucine
mutation

Keywords

  • Eastern India
  • Inter-genogroup reassortment
  • Mutation
  • Wa-like heterogeneous G3 rotavirus
  • Whole genome sequencing

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Upsurge and spread of G3 rotaviruses in Eastern India (2014–2016) : Full genome analyses reveals heterogeneity within Wa-like genomic constellation. / Banerjee, Anindita; Lo, Mahadeb; Indwar, Pallavi; Deb, Alok K.; Das, Santasabuj; Manna, Byomkesh; Dutta, Shanta; Bhadra, Uchhal K.; Bhattacharya, Mala; Okamoto, Keinosuke; Chawla-Sarkar, Mamta.

In: Infection, Genetics and Evolution, Vol. 63, 01.09.2018, p. 158-174.

Research output: Contribution to journalArticle

Banerjee, Anindita ; Lo, Mahadeb ; Indwar, Pallavi ; Deb, Alok K. ; Das, Santasabuj ; Manna, Byomkesh ; Dutta, Shanta ; Bhadra, Uchhal K. ; Bhattacharya, Mala ; Okamoto, Keinosuke ; Chawla-Sarkar, Mamta. / Upsurge and spread of G3 rotaviruses in Eastern India (2014–2016) : Full genome analyses reveals heterogeneity within Wa-like genomic constellation. In: Infection, Genetics and Evolution. 2018 ; Vol. 63. pp. 158-174.
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abstract = "Advent of new strains and shift in predominantly circulating genotypes are characteristics of group- A rotavirus (RVA), one of the major causes of childhood gastroenteritis. During diarrheal disease surveillance at Kolkata, India (2014–2016), a shift in circulating RVA strains from G1P[8] to G3P[8] was seen. Stool samples from children (n = 3048) with acute gastroenteritis were tested of which 38.7{\%} were RVA positive. G1 was the predominant strain (65.3{\%}) in 2014–2015 whereas in late 2015 and 2016, G3 became the preponderant strain (44.6{\%}). In the past decade G3 strains were not observed in this region, we conducted whole genome sequencing of representative strains to gain insight into the phenomenon of emergence and genetic constellation of these circulating human G3 strains. The analyses revealed intergenogroup reassortment in G3P[4] strains (among Wa and DS-1-like genogroup) whereas G3P[8] strains were authentic Wa-like. Phylogenetic analysis revealed Kolkata G3 strains as polymorphic and thus they formed two sub-clusters due to antigenic differences in their VP7 protein. One of the sub-clusters had the wild-type threonine at 87 amino acid position while another sub-cluster had an isoleucine mutation. Presence of additional N-linked glycosylation site at amino acid 283 of VP7 glycoprotein suggests that the major neutralizing epitope on the VP7 (G3) of RotaTeq vaccine differs from the currently circulating G3 strains. The study is important as efficiency of rotavirus vaccine depends on the circulating heterogeneous genotype constellations. Continuous monitoring of circulating RVA strains in endemic settings like India is therefore important in pre- and post-vaccination period to monitor the emergence of new reassortant genotypes in addition to assessing vaccine efficacy.",
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