Upstream element of the sea urchin arylsulfatase gene serves as an insulator.

K. Akasaka, A. Nishimura, K. Takata, K. Mitsunaga, F. Mibuka, Hitoshi Ueda, S. Hirose, K. Tsutsui, H. Shimada

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Insulator DNAs functionally isolate neighboring genes by blocking interactions between distal cis-regulatory elements and promoters. Here we report that a DNA fragment located in the upstream region of sea urchin, H. pulcherrimus, arylsulfatase (HpArs) gene blocks the interaction of the Ars enhancer when positioned between the enhancer and the target promoter, in an orientation dependent manner. The Ars insulator works only 3' to 5' direction and has no significant stimulatory or inhibitory effects on its own promoter. In transgenic Drosophila, the Ars insulator blocks the interaction between even-skipped stripe enhancer and its target promoter. The insulation mechanism operates also unidirectionally in Drosophila. We also show that the efficiency of transformation of HeLa cells is enhanced when the integrated gene is flanked by the Ars insulator, suggesting the sea urchin insulator overcomes the position-dependent transgene expression in mammalian cells. These results demonstrate that the mechanism of action of the insulator has been conserved throughout evolution.

Original languageEnglish
Pages (from-to)555-565
Number of pages11
JournalCellular and Molecular Biology
Volume45
Issue number5
Publication statusPublished - Jul 1999
Externally publishedYes

Fingerprint

Arylsulfatases
Sea Urchins
Genes
Drosophila
DNA
Transgenes
HeLa Cells
Insulation
Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Akasaka, K., Nishimura, A., Takata, K., Mitsunaga, K., Mibuka, F., Ueda, H., ... Shimada, H. (1999). Upstream element of the sea urchin arylsulfatase gene serves as an insulator. Cellular and Molecular Biology, 45(5), 555-565.

Upstream element of the sea urchin arylsulfatase gene serves as an insulator. / Akasaka, K.; Nishimura, A.; Takata, K.; Mitsunaga, K.; Mibuka, F.; Ueda, Hitoshi; Hirose, S.; Tsutsui, K.; Shimada, H.

In: Cellular and Molecular Biology, Vol. 45, No. 5, 07.1999, p. 555-565.

Research output: Contribution to journalArticle

Akasaka, K, Nishimura, A, Takata, K, Mitsunaga, K, Mibuka, F, Ueda, H, Hirose, S, Tsutsui, K & Shimada, H 1999, 'Upstream element of the sea urchin arylsulfatase gene serves as an insulator.', Cellular and Molecular Biology, vol. 45, no. 5, pp. 555-565.
Akasaka K, Nishimura A, Takata K, Mitsunaga K, Mibuka F, Ueda H et al. Upstream element of the sea urchin arylsulfatase gene serves as an insulator. Cellular and Molecular Biology. 1999 Jul;45(5):555-565.
Akasaka, K. ; Nishimura, A. ; Takata, K. ; Mitsunaga, K. ; Mibuka, F. ; Ueda, Hitoshi ; Hirose, S. ; Tsutsui, K. ; Shimada, H. / Upstream element of the sea urchin arylsulfatase gene serves as an insulator. In: Cellular and Molecular Biology. 1999 ; Vol. 45, No. 5. pp. 555-565.
@article{6821abaa8f3c46339d1fcdde664fbfa2,
title = "Upstream element of the sea urchin arylsulfatase gene serves as an insulator.",
abstract = "Insulator DNAs functionally isolate neighboring genes by blocking interactions between distal cis-regulatory elements and promoters. Here we report that a DNA fragment located in the upstream region of sea urchin, H. pulcherrimus, arylsulfatase (HpArs) gene blocks the interaction of the Ars enhancer when positioned between the enhancer and the target promoter, in an orientation dependent manner. The Ars insulator works only 3' to 5' direction and has no significant stimulatory or inhibitory effects on its own promoter. In transgenic Drosophila, the Ars insulator blocks the interaction between even-skipped stripe enhancer and its target promoter. The insulation mechanism operates also unidirectionally in Drosophila. We also show that the efficiency of transformation of HeLa cells is enhanced when the integrated gene is flanked by the Ars insulator, suggesting the sea urchin insulator overcomes the position-dependent transgene expression in mammalian cells. These results demonstrate that the mechanism of action of the insulator has been conserved throughout evolution.",
author = "K. Akasaka and A. Nishimura and K. Takata and K. Mitsunaga and F. Mibuka and Hitoshi Ueda and S. Hirose and K. Tsutsui and H. Shimada",
year = "1999",
month = "7",
language = "English",
volume = "45",
pages = "555--565",
journal = "Cellular and Molecular Biology",
issn = "0145-5680",
publisher = "Cellular and Molecular Biology Association",
number = "5",

}

TY - JOUR

T1 - Upstream element of the sea urchin arylsulfatase gene serves as an insulator.

AU - Akasaka, K.

AU - Nishimura, A.

AU - Takata, K.

AU - Mitsunaga, K.

AU - Mibuka, F.

AU - Ueda, Hitoshi

AU - Hirose, S.

AU - Tsutsui, K.

AU - Shimada, H.

PY - 1999/7

Y1 - 1999/7

N2 - Insulator DNAs functionally isolate neighboring genes by blocking interactions between distal cis-regulatory elements and promoters. Here we report that a DNA fragment located in the upstream region of sea urchin, H. pulcherrimus, arylsulfatase (HpArs) gene blocks the interaction of the Ars enhancer when positioned between the enhancer and the target promoter, in an orientation dependent manner. The Ars insulator works only 3' to 5' direction and has no significant stimulatory or inhibitory effects on its own promoter. In transgenic Drosophila, the Ars insulator blocks the interaction between even-skipped stripe enhancer and its target promoter. The insulation mechanism operates also unidirectionally in Drosophila. We also show that the efficiency of transformation of HeLa cells is enhanced when the integrated gene is flanked by the Ars insulator, suggesting the sea urchin insulator overcomes the position-dependent transgene expression in mammalian cells. These results demonstrate that the mechanism of action of the insulator has been conserved throughout evolution.

AB - Insulator DNAs functionally isolate neighboring genes by blocking interactions between distal cis-regulatory elements and promoters. Here we report that a DNA fragment located in the upstream region of sea urchin, H. pulcherrimus, arylsulfatase (HpArs) gene blocks the interaction of the Ars enhancer when positioned between the enhancer and the target promoter, in an orientation dependent manner. The Ars insulator works only 3' to 5' direction and has no significant stimulatory or inhibitory effects on its own promoter. In transgenic Drosophila, the Ars insulator blocks the interaction between even-skipped stripe enhancer and its target promoter. The insulation mechanism operates also unidirectionally in Drosophila. We also show that the efficiency of transformation of HeLa cells is enhanced when the integrated gene is flanked by the Ars insulator, suggesting the sea urchin insulator overcomes the position-dependent transgene expression in mammalian cells. These results demonstrate that the mechanism of action of the insulator has been conserved throughout evolution.

UR - http://www.scopus.com/inward/record.url?scp=0033155772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033155772&partnerID=8YFLogxK

M3 - Article

VL - 45

SP - 555

EP - 565

JO - Cellular and Molecular Biology

JF - Cellular and Molecular Biology

SN - 0145-5680

IS - 5

ER -