Upregulation of protein-tyrosine nitration in the anterior horn cells of amyotrophic lateral sclerosis

K. Abe, L. H. Pan, M. Watanabe, H. Konno, T. Kato, Y. Itoyama

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Spinal cords of sporadic cases with amyotrophic lateral sclerosis (ALS) and normal controls were immunohistochemically examined using antibodies for nitrotyrosine (NT), Cu/Zn superoxide dismutase (SOD), and nitric oxide synthase (NOS) of brain, endothelial, and inducible forms. Immunoreactivity for NT was densely detected in the motor neurons of ALS while it was not or was only minimally detected in those of controls. The staining was also found in the axons of motor neurons of ALS, but was not found in the controls. In contrast, although immunoreactivity for Cu/Zn SOD of the motor neurons was dense in the motor neurons, it was not different between the ALS and controls. Immunoreactivities for bNOS and eNOS in the motor neurons of ALS were stronger than those of controls, and were also found in degenerated axons in the anterior horn of ALS. However, the immunoreactivity for inducible NOS was only minimally detected in the motor neurons of ALS and controls, and was not detected in the degenerated axons of ALS. These results suggest that nitration of protein-tyrosine residue is upregulated in motor neurons of the spinal cord of ALS with selective increases of brain NOS- and endothelial NOS-like immunoreactivities.

Original languageEnglish
Pages (from-to)124-128
Number of pages5
JournalNeurological Research
Volume19
Issue number2
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

Keywords

  • Nitrotyrosine
  • amyotrophic lateral sclerosis
  • nitric oxide synthase
  • superoxide dismutase

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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