Abstract
The unfolded protein response (UPR) handles unfolded/misfolded proteins accumulated in the endoplasmic reticulum (ER). However, it is unclear how vertebrates correctly use the total of ten UPR transducers. We have found that ER stress occurs physiologically during early embryonic development in medaka fish and that the smooth alignment of notochord cells requires ATF6 as a UPR transducer, which induces ER chaperones for folding of type VIII (short-chain) collagen. After secretion of hedgehog for tissue patterning, notochord cells differentiate into sheath cells, which synthesize type II collagen. In this study, we show that this vacuolization step requires both ATF6 and BBF2H7 as UPR transducers and that BBF2H7 regulates a complete set of genes (Sec23/24/13/31, Tango1, Sedlin, and KLHL12) essential for the enlargement of COP II vesicles to accommodate long-chain collagen for export, leading to the formation of the perinotochordal basement membrane. Thus, the most appropriate UPR transducer is activated to cope with the differing physiological ER stresses of different content types depending on developmental stage.
| Original language | English |
|---|---|
| Pages (from-to) | 1761-1774 |
| Number of pages | 14 |
| Journal | Journal of Cell Biology |
| Volume | 216 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 1 2017 |
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ASJC Scopus subject areas
- Cell Biology
Cite this
UPR transducer BBF2H7 allows export of type II collagen in a cargo- and developmental stage- specific manner. / Ishikawa, Tokiro; Toyama, Takuya; Nakamura, Yuki; Tamada, Kentaro; Shimizu, Hitomi; Ninagawa, Satoshi; Okada, Tetsuya; Kamei, Yasuhiro; Ishikawa-Fujiwara, Tomoko; Todo, Takeshi; Aoyama, Eriko; Takigawa, Masaharu; Harada, Akihiro; Mori, Kazutoshi.
In: Journal of Cell Biology, Vol. 216, No. 6, 01.06.2017, p. 1761-1774.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - UPR transducer BBF2H7 allows export of type II collagen in a cargo- and developmental stage- specific manner
AU - Ishikawa, Tokiro
AU - Toyama, Takuya
AU - Nakamura, Yuki
AU - Tamada, Kentaro
AU - Shimizu, Hitomi
AU - Ninagawa, Satoshi
AU - Okada, Tetsuya
AU - Kamei, Yasuhiro
AU - Ishikawa-Fujiwara, Tomoko
AU - Todo, Takeshi
AU - Aoyama, Eriko
AU - Takigawa, Masaharu
AU - Harada, Akihiro
AU - Mori, Kazutoshi
PY - 2017/6/1
Y1 - 2017/6/1
N2 - The unfolded protein response (UPR) handles unfolded/misfolded proteins accumulated in the endoplasmic reticulum (ER). However, it is unclear how vertebrates correctly use the total of ten UPR transducers. We have found that ER stress occurs physiologically during early embryonic development in medaka fish and that the smooth alignment of notochord cells requires ATF6 as a UPR transducer, which induces ER chaperones for folding of type VIII (short-chain) collagen. After secretion of hedgehog for tissue patterning, notochord cells differentiate into sheath cells, which synthesize type II collagen. In this study, we show that this vacuolization step requires both ATF6 and BBF2H7 as UPR transducers and that BBF2H7 regulates a complete set of genes (Sec23/24/13/31, Tango1, Sedlin, and KLHL12) essential for the enlargement of COP II vesicles to accommodate long-chain collagen for export, leading to the formation of the perinotochordal basement membrane. Thus, the most appropriate UPR transducer is activated to cope with the differing physiological ER stresses of different content types depending on developmental stage.
AB - The unfolded protein response (UPR) handles unfolded/misfolded proteins accumulated in the endoplasmic reticulum (ER). However, it is unclear how vertebrates correctly use the total of ten UPR transducers. We have found that ER stress occurs physiologically during early embryonic development in medaka fish and that the smooth alignment of notochord cells requires ATF6 as a UPR transducer, which induces ER chaperones for folding of type VIII (short-chain) collagen. After secretion of hedgehog for tissue patterning, notochord cells differentiate into sheath cells, which synthesize type II collagen. In this study, we show that this vacuolization step requires both ATF6 and BBF2H7 as UPR transducers and that BBF2H7 regulates a complete set of genes (Sec23/24/13/31, Tango1, Sedlin, and KLHL12) essential for the enlargement of COP II vesicles to accommodate long-chain collagen for export, leading to the formation of the perinotochordal basement membrane. Thus, the most appropriate UPR transducer is activated to cope with the differing physiological ER stresses of different content types depending on developmental stage.
UR - http://www.scopus.com/inward/record.url?scp=85021797146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85021797146&partnerID=8YFLogxK
U2 - 10.1083/jcb.201609100
DO - 10.1083/jcb.201609100
M3 - Article
VL - 216
SP - 1761
EP - 1774
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 6
ER -