Up-regulation of the peroxisomal β-oxidation system occurs in butyrate-grown Candida tropicalis following disruption of the gene encoding peroxisomal 3-ketoacyl-CoA thiolase

Mitsuyoshi Ueda, Naoki Kanayama, Naomi Kamasawa, Masako Osumi, Atsuo Tanaka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the yeast Candida tropicalis, two thiolase isozymes, peroxisomal acetoacetyl-CoA thiolase and peroxisomal 3-ketoacyl-CoA thiolase, participate in the peroxisomal fatty acid β-oxidation system. Their individual contributions have been demonstrated in cells grown on butyrate, with C. tropicalis able to grow in the absence of either one. In the present study, a lack of peroxisomal 3-ketoacyl-CoA thiolase protein resulted in increased expression (up-regulation) of acetoacetyl-CoA thiolase and other peroxisomal proteins, whereas a lack of peroxisomal acetoacetyl-CoA thiolase produced no corresponding effect. Overexpression of the acetoacetyl-CoA thiolase gene did not suppress the up-regulation or the growth retardation on butyrate in cells without peroxisomal 3-ketoacyl-CoA thiolase, even though large amounts of the overexpressed acetoacetyl-CoA thiolase were detected in most of the peroxisomes of butyrate-grown cells. These results provide important evidence of the greater contribution of 3-ketoacyl-CoA thiolase to the peroxisomal β-oxidation system than acetoacetyl-CoA thiolase in C. tropicalis and a novel insight into the regulation of the peroxisomal β-oxidation system.

Original languageEnglish
Pages (from-to)160-168
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1631
Issue number2
DOIs
Publication statusPublished - Mar 17 2003
Externally publishedYes

Keywords

  • Butyrate
  • Cytosolic acetoacetyl-CoA thiolase
  • Peroxisomal 3-ketoacyl-CoA thiolase
  • Peroxisomal acetoacetyl-CoA thiolase
  • Peroxisomal fatty acid β-oxidation
  • Yeast Candida tropicalis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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