Abstract
In the yeast Candida tropicalis, two thiolase isozymes, peroxisomal acetoacetyl-CoA thiolase and peroxisomal 3-ketoacyl-CoA thiolase, participate in the peroxisomal fatty acid β-oxidation system. Their individual contributions have been demonstrated in cells grown on butyrate, with C. tropicalis able to grow in the absence of either one. In the present study, a lack of peroxisomal 3-ketoacyl-CoA thiolase protein resulted in increased expression (up-regulation) of acetoacetyl-CoA thiolase and other peroxisomal proteins, whereas a lack of peroxisomal acetoacetyl-CoA thiolase produced no corresponding effect. Overexpression of the acetoacetyl-CoA thiolase gene did not suppress the up-regulation or the growth retardation on butyrate in cells without peroxisomal 3-ketoacyl-CoA thiolase, even though large amounts of the overexpressed acetoacetyl-CoA thiolase were detected in most of the peroxisomes of butyrate-grown cells. These results provide important evidence of the greater contribution of 3-ketoacyl-CoA thiolase to the peroxisomal β-oxidation system than acetoacetyl-CoA thiolase in C. tropicalis and a novel insight into the regulation of the peroxisomal β-oxidation system.
Original language | English |
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Pages (from-to) | 160-168 |
Number of pages | 9 |
Journal | Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids |
Volume | 1631 |
Issue number | 2 |
DOIs | |
Publication status | Published - Mar 17 2003 |
Externally published | Yes |
Keywords
- Butyrate
- Cytosolic acetoacetyl-CoA thiolase
- Peroxisomal 3-ketoacyl-CoA thiolase
- Peroxisomal acetoacetyl-CoA thiolase
- Peroxisomal fatty acid β-oxidation
- Yeast Candida tropicalis
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology