Up-regulation of low-density lipoprotein receptor expression in the ischemic core and the peri-ischemic area after transient MCA occlusion in rats

Hiroshi Kamada, Takeshi Hayashi, Keiko Sato, Masanori Iwai, Isao Nagano, Mikio Shoji, Koji Abe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Low-density lipoprotein (LDL) receptor is involved in cholesterol metabolism of CNS as a receptor of apolipoprotein E (ApoE), which plays an important role in regenerative process after brain ischemia. Temporal and spatial changes of LDL receptor were investigated after 90 min of transient middle cerebral artery occlusion (MCAO) in relation to those of microtubule-associated protein 2 (MAP2) and ApoE. In the ischemic core, LDL receptor became positive at 1 d after transient MCAO, which was not double positive for MAP2 or ApoE, and disappeared in 7 and 56 d. In the peri-ischemic area, LDL receptor became observed at 7 d, which peaked at 21 d, most of which were double positive for MAP2. The number of LDL receptor and ApoE double-positive cells increased at 7 d and decreased at 21 d with the shift of LDL receptor immunoreactivity from cytoplasm at 7 d to dendrites at 21 d in the peri-ischemic area. These results suggest that LDL receptor, interacting with ApoE, is profoundly involved in lipid transport of CNS for tissue repair in the peri-ischemic area after brain ischemia.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalMolecular Brain Research
Volume134
Issue number2
DOIs
Publication statusPublished - Apr 4 2005

Keywords

  • ApoE
  • Brain ischemia
  • LDL receptor
  • Lipid
  • Rat

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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