TY - JOUR
T1 - Up-regulation of low-density lipoprotein receptor expression in the ischemic core and the peri-ischemic area after transient MCA occlusion in rats
AU - Kamada, Hiroshi
AU - Hayashi, Takeshi
AU - Sato, Keiko
AU - Iwai, Masanori
AU - Nagano, Isao
AU - Shoji, Mikio
AU - Abe, Koji
N1 - Funding Information:
This work was partly supported by Grant-in Aid for Scientific Research (B) 15390273, (Hoga) 15659338, and National Project on Protein Structural and Functional Analyses from the Ministry of Education, Science, Culture and Sports of Japan, and grants (Itoyama Y, Kimura I and Kuzuhara S) from the Ministry of Health and Welfare of Japan.
PY - 2005/4/4
Y1 - 2005/4/4
N2 - Low-density lipoprotein (LDL) receptor is involved in cholesterol metabolism of CNS as a receptor of apolipoprotein E (ApoE), which plays an important role in regenerative process after brain ischemia. Temporal and spatial changes of LDL receptor were investigated after 90 min of transient middle cerebral artery occlusion (MCAO) in relation to those of microtubule-associated protein 2 (MAP2) and ApoE. In the ischemic core, LDL receptor became positive at 1 d after transient MCAO, which was not double positive for MAP2 or ApoE, and disappeared in 7 and 56 d. In the peri-ischemic area, LDL receptor became observed at 7 d, which peaked at 21 d, most of which were double positive for MAP2. The number of LDL receptor and ApoE double-positive cells increased at 7 d and decreased at 21 d with the shift of LDL receptor immunoreactivity from cytoplasm at 7 d to dendrites at 21 d in the peri-ischemic area. These results suggest that LDL receptor, interacting with ApoE, is profoundly involved in lipid transport of CNS for tissue repair in the peri-ischemic area after brain ischemia.
AB - Low-density lipoprotein (LDL) receptor is involved in cholesterol metabolism of CNS as a receptor of apolipoprotein E (ApoE), which plays an important role in regenerative process after brain ischemia. Temporal and spatial changes of LDL receptor were investigated after 90 min of transient middle cerebral artery occlusion (MCAO) in relation to those of microtubule-associated protein 2 (MAP2) and ApoE. In the ischemic core, LDL receptor became positive at 1 d after transient MCAO, which was not double positive for MAP2 or ApoE, and disappeared in 7 and 56 d. In the peri-ischemic area, LDL receptor became observed at 7 d, which peaked at 21 d, most of which were double positive for MAP2. The number of LDL receptor and ApoE double-positive cells increased at 7 d and decreased at 21 d with the shift of LDL receptor immunoreactivity from cytoplasm at 7 d to dendrites at 21 d in the peri-ischemic area. These results suggest that LDL receptor, interacting with ApoE, is profoundly involved in lipid transport of CNS for tissue repair in the peri-ischemic area after brain ischemia.
KW - ApoE
KW - Brain ischemia
KW - LDL receptor
KW - Lipid
KW - Rat
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U2 - 10.1016/j.molbrainres.2004.10.037
DO - 10.1016/j.molbrainres.2004.10.037
M3 - Article
C2 - 15836915
AN - SCOPUS:17044368304
VL - 134
SP - 181
EP - 188
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -