Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum

Masayuki Sakurai, Takashi Ohtsuki, Tsutomu Suzuki, Kimitsuna Watanabe

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm5U) was identified at the wobble positions of tRNA Lys, tRNAGlu and tRNAGln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm5s2U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.

Original languageEnglish
Pages (from-to)2767-2772
Number of pages6
JournalFEBS Letters
Volume579
Issue number13
DOIs
Publication statusPublished - May 23 2005

Keywords

  • 2-Thiouridine
  • Mitochondrial tRNA
  • Modified nucleoside
  • Nematode
  • T-armless tRNA
  • Wobble position

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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