Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum

Masayuki Sakurai; Takashi Ohtsuki; Tsutomu Suzuki; Kimitsuna Watanabe

doi:10.1016/j.febslet.2005.04.009

Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum

Masayuki Sakurai, Takashi Ohtsuki, Tsutomu Suzuki, Kimitsuna Watanabe

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm⁵U) was identified at the wobble positions of tRNA ^Lys, tRNA^Glu and tRNA^Gln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm⁵s²U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm⁵s²U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.

Original language	English
Pages (from-to)	2767-2772
Number of pages	6
Journal	FEBS Letters
Volume	579
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2005.04.009
Publication status	Published - May 23 2005

Keywords

2-Thiouridine
Mitochondrial tRNA
Modified nucleoside
Nematode
T-armless tRNA
Wobble position

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum",

abstract = "To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm5U) was identified at the wobble positions of tRNA Lys, tRNAGlu and tRNAGln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm5s2U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.",

keywords = "2-Thiouridine, Mitochondrial tRNA, Modified nucleoside, Nematode, T-armless tRNA, Wobble position",

author = "Masayuki Sakurai and Takashi Ohtsuki and Tsutomu Suzuki and Kimitsuna Watanabe",

note = "Funding Information: This work was supported by Grants-in-Aid from the Ministry of Culture, Sports, Science and Technology, Japan to K.W. and T.O. We thank Dr. Y. Watanabe for materials and valuable discussions, and Dr. K. Kita, Dr. N. Shigi and Mr. Y. Yamamoto for materials. We are grateful to Dr. Takeo Suzuki and Mr. Y. Ikeuchi for their technical assistance on tRNA isolation and mass spectrometry. ",

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doi = "10.1016/j.febslet.2005.04.009",

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pages = "2767--2772",

journal = "FEBS Letters",

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AU - Sakurai, Masayuki

AU - Ohtsuki, Takashi

AU - Suzuki, Tsutomu

AU - Watanabe, Kimitsuna

N1 - Funding Information: This work was supported by Grants-in-Aid from the Ministry of Culture, Sports, Science and Technology, Japan to K.W. and T.O. We thank Dr. Y. Watanabe for materials and valuable discussions, and Dr. K. Kita, Dr. N. Shigi and Mr. Y. Yamamoto for materials. We are grateful to Dr. Takeo Suzuki and Mr. Y. Ikeuchi for their technical assistance on tRNA isolation and mass spectrometry.

PY - 2005/5/23

Y1 - 2005/5/23

N2 - To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm5U) was identified at the wobble positions of tRNA Lys, tRNAGlu and tRNAGln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm5s2U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.

AB - To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm5U) was identified at the wobble positions of tRNA Lys, tRNAGlu and tRNAGln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm5s2U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.

KW - 2-Thiouridine

KW - Mitochondrial tRNA

KW - Modified nucleoside

KW - Nematode

KW - T-armless tRNA

KW - Wobble position

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