Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt-induced damage in Synechococcus

Suleyman Allakhverdiev, M. Kinoshita, M. Inaba, I. Suzuki, N. Murata

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

In this study, the tolerance to salt stress of the photosynthetic machinery was examined in relation to the effects of the genetic enhancement of the unsaturation of fatty acids in membrane lipids in wild-type and desA+ cells of Synechococcus sp. PCC 7942. Wild-type cells synthesized saturated and mono-unsaturated fatty acids, whereas desA+ cells, which had been transformed with the desA gene for the Δ12 acyl-lipid desaturase of Synechocystis sp. PCC 6803, also synthesized di-unsaturated fatty acids. Incubation of wild-type and desA+ cells with 0.5 M NaCl resulted in the rapid loss of the activities of photosystem I, photosystem II, and the Na+/H+ antiport system both in light and in darkness. However, desA+ cells were more tolerant to salt stress and osmotic stress than the wild-type cells. The extent of the recovery of the various photosynthetic activities from the effects of 0.5 M NaCl was much greater in desA+ cells than in wild-type cells. The photosystem II activity of thylakoid membranes from desA+ cells was more resistant to 0.5 M NaCl than that of membranes from wild-type cells. These results demonstrated that the genetically engineered increase in unsaturation of fatty acids in membrane lipids significantly enhanced the tolerance of the photosynthetic machinery to salt stress. The enhanced tolerance was due both to the increased resistance of the photosynthetic machinery to the salt-induced damage and to the increased ability of desA+ cells to repair the photosynthetic and Na+/H+ antiport systems.

Original languageEnglish
Pages (from-to)1842-1853
Number of pages12
JournalPlant Physiology
Volume125
Issue number4
DOIs
Publication statusPublished - May 8 2001
Externally publishedYes

Fingerprint

Synechococcus
Membrane Lipids
Unsaturated Fatty Acids
unsaturated fatty acids
Salts
salts
lipids
cells
salt stress
Photosystem II Protein Complex
Ion Transport
photosystem II
Genetic Enhancement
Fatty Acids
Synechococcus sp. PCC 7942
Synechocystis
Photosystem I Protein Complex
fatty acids
Thylakoids
Synechocystis sp. PCC 6803

ASJC Scopus subject areas

  • Plant Science

Cite this

Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt-induced damage in Synechococcus. / Allakhverdiev, Suleyman; Kinoshita, M.; Inaba, M.; Suzuki, I.; Murata, N.

In: Plant Physiology, Vol. 125, No. 4, 08.05.2001, p. 1842-1853.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Kinoshita, M. ; Inaba, M. ; Suzuki, I. ; Murata, N. / Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt-induced damage in Synechococcus. In: Plant Physiology. 2001 ; Vol. 125, No. 4. pp. 1842-1853.
@article{12608b36cce44915970c6ea53a569514,
title = "Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt-induced damage in Synechococcus",
abstract = "In this study, the tolerance to salt stress of the photosynthetic machinery was examined in relation to the effects of the genetic enhancement of the unsaturation of fatty acids in membrane lipids in wild-type and desA+ cells of Synechococcus sp. PCC 7942. Wild-type cells synthesized saturated and mono-unsaturated fatty acids, whereas desA+ cells, which had been transformed with the desA gene for the Δ12 acyl-lipid desaturase of Synechocystis sp. PCC 6803, also synthesized di-unsaturated fatty acids. Incubation of wild-type and desA+ cells with 0.5 M NaCl resulted in the rapid loss of the activities of photosystem I, photosystem II, and the Na+/H+ antiport system both in light and in darkness. However, desA+ cells were more tolerant to salt stress and osmotic stress than the wild-type cells. The extent of the recovery of the various photosynthetic activities from the effects of 0.5 M NaCl was much greater in desA+ cells than in wild-type cells. The photosystem II activity of thylakoid membranes from desA+ cells was more resistant to 0.5 M NaCl than that of membranes from wild-type cells. These results demonstrated that the genetically engineered increase in unsaturation of fatty acids in membrane lipids significantly enhanced the tolerance of the photosynthetic machinery to salt stress. The enhanced tolerance was due both to the increased resistance of the photosynthetic machinery to the salt-induced damage and to the increased ability of desA+ cells to repair the photosynthetic and Na+/H+ antiport systems.",
author = "Suleyman Allakhverdiev and M. Kinoshita and M. Inaba and I. Suzuki and N. Murata",
year = "2001",
month = "5",
day = "8",
doi = "10.1104/pp.125.4.1842",
language = "English",
volume = "125",
pages = "1842--1853",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

TY - JOUR

T1 - Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt-induced damage in Synechococcus

AU - Allakhverdiev, Suleyman

AU - Kinoshita, M.

AU - Inaba, M.

AU - Suzuki, I.

AU - Murata, N.

PY - 2001/5/8

Y1 - 2001/5/8

N2 - In this study, the tolerance to salt stress of the photosynthetic machinery was examined in relation to the effects of the genetic enhancement of the unsaturation of fatty acids in membrane lipids in wild-type and desA+ cells of Synechococcus sp. PCC 7942. Wild-type cells synthesized saturated and mono-unsaturated fatty acids, whereas desA+ cells, which had been transformed with the desA gene for the Δ12 acyl-lipid desaturase of Synechocystis sp. PCC 6803, also synthesized di-unsaturated fatty acids. Incubation of wild-type and desA+ cells with 0.5 M NaCl resulted in the rapid loss of the activities of photosystem I, photosystem II, and the Na+/H+ antiport system both in light and in darkness. However, desA+ cells were more tolerant to salt stress and osmotic stress than the wild-type cells. The extent of the recovery of the various photosynthetic activities from the effects of 0.5 M NaCl was much greater in desA+ cells than in wild-type cells. The photosystem II activity of thylakoid membranes from desA+ cells was more resistant to 0.5 M NaCl than that of membranes from wild-type cells. These results demonstrated that the genetically engineered increase in unsaturation of fatty acids in membrane lipids significantly enhanced the tolerance of the photosynthetic machinery to salt stress. The enhanced tolerance was due both to the increased resistance of the photosynthetic machinery to the salt-induced damage and to the increased ability of desA+ cells to repair the photosynthetic and Na+/H+ antiport systems.

AB - In this study, the tolerance to salt stress of the photosynthetic machinery was examined in relation to the effects of the genetic enhancement of the unsaturation of fatty acids in membrane lipids in wild-type and desA+ cells of Synechococcus sp. PCC 7942. Wild-type cells synthesized saturated and mono-unsaturated fatty acids, whereas desA+ cells, which had been transformed with the desA gene for the Δ12 acyl-lipid desaturase of Synechocystis sp. PCC 6803, also synthesized di-unsaturated fatty acids. Incubation of wild-type and desA+ cells with 0.5 M NaCl resulted in the rapid loss of the activities of photosystem I, photosystem II, and the Na+/H+ antiport system both in light and in darkness. However, desA+ cells were more tolerant to salt stress and osmotic stress than the wild-type cells. The extent of the recovery of the various photosynthetic activities from the effects of 0.5 M NaCl was much greater in desA+ cells than in wild-type cells. The photosystem II activity of thylakoid membranes from desA+ cells was more resistant to 0.5 M NaCl than that of membranes from wild-type cells. These results demonstrated that the genetically engineered increase in unsaturation of fatty acids in membrane lipids significantly enhanced the tolerance of the photosynthetic machinery to salt stress. The enhanced tolerance was due both to the increased resistance of the photosynthetic machinery to the salt-induced damage and to the increased ability of desA+ cells to repair the photosynthetic and Na+/H+ antiport systems.

UR - http://www.scopus.com/inward/record.url?scp=0035028257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035028257&partnerID=8YFLogxK

U2 - 10.1104/pp.125.4.1842

DO - 10.1104/pp.125.4.1842

M3 - Article

C2 - 11299364

AN - SCOPUS:0035028257

VL - 125

SP - 1842

EP - 1853

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -