Unnatural base pairs for specific transcription

Takashi Ohtsuki, Michiko Kimoto, Masahide Ishikawa, Tsuneo Mitsui, Ichiro Hirao, Shigeyuki Yokoyama

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

An unnatural base pair of 2-amino-6-(N,N-dimethylamino)purine (designated as x) and pyridin-2-one (designated as y) has been developed for specific transcription. The ribonucleoside triphosphates of y and a modified y, 5-methylpyridin-2-one, are selectively incorporated into RNA opposite x in the templates by T7 RNA polymerase. In addition, the sequences of the DNA templates containing x can be confirmed by a dideoxynucleotide chainterminator method supplemented with the deoxynucleoside triphosphate of y. The bulky dimethylamino group of x in the templates effectively eliminates noncognate pairing with the natural bases. These results enable RNA biosynthesis for the specific incorporation of unnatural nucleotides at the desired positions.

Original languageEnglish
Pages (from-to)4922-4925
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number9
DOIs
Publication statusPublished - Apr 24 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • General

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