Abstract
The unnatural base, 2-amino-6-(2-thienyl)purine (designated as s), instead of 2-amino-6-(N,N-dimethylamino)purine (designated as x), was designed in order to improve the specificity and efficiency of the base pairing with pyridin-2-one (designated as y). DNA fragments containing s were chemically synthesized, and the thermal stability and the enzymatic reactions involving the s-y pairing were examined. Thermal denaturation experiments showed that the DNA duplex (12-mer) containing the s-y pair was more stable than that containing the x-y pair. The incorporation of dyTP was also more advantageous to the s-y pairing than the x-y pairing in single-nucleotide insertion experiments using the Klenow fragment of Escherichia coli DNA polymerase I.
| Original language | English |
|---|---|
| Pages (from-to) | 261-262 |
| Number of pages | 2 |
| Journal | Nucleic acids symposium series |
| Issue number | 44 |
| Publication status | Published - 2000 |
| Externally published | Yes |
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Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases. / Hirao, I.; Fujiwara, T.; Kimoto, M.; Mitsui, T.; Okuni, T.; Ohtsuki, Takashi; Yokoyama, S.
In: Nucleic acids symposium series, No. 44, 2000, p. 261-262.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases.
AU - Hirao, I.
AU - Fujiwara, T.
AU - Kimoto, M.
AU - Mitsui, T.
AU - Okuni, T.
AU - Ohtsuki, Takashi
AU - Yokoyama, S.
PY - 2000
Y1 - 2000
N2 - The unnatural base, 2-amino-6-(2-thienyl)purine (designated as s), instead of 2-amino-6-(N,N-dimethylamino)purine (designated as x), was designed in order to improve the specificity and efficiency of the base pairing with pyridin-2-one (designated as y). DNA fragments containing s were chemically synthesized, and the thermal stability and the enzymatic reactions involving the s-y pairing were examined. Thermal denaturation experiments showed that the DNA duplex (12-mer) containing the s-y pair was more stable than that containing the x-y pair. The incorporation of dyTP was also more advantageous to the s-y pairing than the x-y pairing in single-nucleotide insertion experiments using the Klenow fragment of Escherichia coli DNA polymerase I.
AB - The unnatural base, 2-amino-6-(2-thienyl)purine (designated as s), instead of 2-amino-6-(N,N-dimethylamino)purine (designated as x), was designed in order to improve the specificity and efficiency of the base pairing with pyridin-2-one (designated as y). DNA fragments containing s were chemically synthesized, and the thermal stability and the enzymatic reactions involving the s-y pairing were examined. Thermal denaturation experiments showed that the DNA duplex (12-mer) containing the s-y pair was more stable than that containing the x-y pair. The incorporation of dyTP was also more advantageous to the s-y pairing than the x-y pairing in single-nucleotide insertion experiments using the Klenow fragment of Escherichia coli DNA polymerase I.
UR - http://www.scopus.com/inward/record.url?scp=0141782150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141782150&partnerID=8YFLogxK
M3 - Article
SP - 261
EP - 262
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
SN - 0261-3166
IS - 44
ER -