Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases.

I. Hirao, T. Fujiwara, M. Kimoto, T. Mitsui, T. Okuni, Takashi Ohtsuki, S. Yokoyama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The unnatural base, 2-amino-6-(2-thienyl)purine (designated as s), instead of 2-amino-6-(N,N-dimethylamino)purine (designated as x), was designed in order to improve the specificity and efficiency of the base pairing with pyridin-2-one (designated as y). DNA fragments containing s were chemically synthesized, and the thermal stability and the enzymatic reactions involving the s-y pairing were examined. Thermal denaturation experiments showed that the DNA duplex (12-mer) containing the s-y pair was more stable than that containing the x-y pair. The incorporation of dyTP was also more advantageous to the s-y pairing than the x-y pairing in single-nucleotide insertion experiments using the Klenow fragment of Escherichia coli DNA polymerase I.

Original languageEnglish
Pages (from-to)261-262
Number of pages2
JournalNucleic acids symposium series
Issue number44
Publication statusPublished - 2000
Externally publishedYes

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DNA Polymerase I
Base Pairing
Hot Temperature
DNA
Nucleotides
Escherichia coli
2-amino-6-(2-thienyl)purine
2-amino-6-(N,N-dimethylamino)purine

Cite this

Hirao, I., Fujiwara, T., Kimoto, M., Mitsui, T., Okuni, T., Ohtsuki, T., & Yokoyama, S. (2000). Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases. Nucleic acids symposium series, (44), 261-262.

Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases. / Hirao, I.; Fujiwara, T.; Kimoto, M.; Mitsui, T.; Okuni, T.; Ohtsuki, Takashi; Yokoyama, S.

In: Nucleic acids symposium series, No. 44, 2000, p. 261-262.

Research output: Contribution to journalArticle

Hirao, I, Fujiwara, T, Kimoto, M, Mitsui, T, Okuni, T, Ohtsuki, T & Yokoyama, S 2000, 'Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases.', Nucleic acids symposium series, no. 44, pp. 261-262.
Hirao, I. ; Fujiwara, T. ; Kimoto, M. ; Mitsui, T. ; Okuni, T. ; Ohtsuki, Takashi ; Yokoyama, S. / Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases. In: Nucleic acids symposium series. 2000 ; No. 44. pp. 261-262.
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