Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases.

I. Hirao, T. Fujiwara, M. Kimoto, T. Mitsui, T. Okuni, Takashi Ohtsuki, S. Yokoyama

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Abstract

The unnatural base, 2-amino-6-(2-thienyl)purine (designated as s), instead of 2-amino-6-(N,N-dimethylamino)purine (designated as x), was designed in order to improve the specificity and efficiency of the base pairing with pyridin-2-one (designated as y). DNA fragments containing s were chemically synthesized, and the thermal stability and the enzymatic reactions involving the s-y pairing were examined. Thermal denaturation experiments showed that the DNA duplex (12-mer) containing the s-y pair was more stable than that containing the x-y pair. The incorporation of dyTP was also more advantageous to the s-y pairing than the x-y pairing in single-nucleotide insertion experiments using the Klenow fragment of Escherichia coli DNA polymerase I.

Original languageEnglish
Pages (from-to)261-262
Number of pages2
JournalNucleic acids symposium series
Issue number44
Publication statusPublished - 2000
Externally publishedYes

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Hirao, I., Fujiwara, T., Kimoto, M., Mitsui, T., Okuni, T., Ohtsuki, T., & Yokoyama, S. (2000). Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases. Nucleic acids symposium series, (44), 261-262.