Unique mutation portraits and frequent COL2A1 gene alteration in chondrosarcoma

Aki Yoshida, Yasushi Totoki, Akihiko Yoshida, Fumie Hosoda, Hiromi Nakamura, Natsuko Hama, Koichi Ogura, Tomohiro Fujiwara, Yasuhito Arai, Junya Toguchida, Hitoshi Tsuda, Satoru Miyano, Akira Kawai, Tatsuhiro Shibata

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Chondrosarcoma is the second most frequent malignant bone tumor. However, the etiological background of chondrosarcomagenesis remains largely unknown, along with details on molecular alterations and potential therapeutic targets. Massively parallel paired-end sequencing of whole genomes of 10 primary chondrosarcomas revealed that the process of accumulation of somatic mutations is homogeneous irrespective of the pathological subtype or the presence of IDH1 mutations, is unique among a range of cancer types, and shares significant commonalities with that of prostate cancer. Clusters of structural alterations localized within a single chromosome were observed in four cases. Combined with targeted resequencing of additional cartilaginous tumor cohorts, we identified somatic alterations of the COL2A1 gene, which encodes an essential extracellular matrix protein in chondroskeletal development, in 19.3% of chondrosarcoma and 31.7% of enchondroma cases. Epigenetic regulators (IDH1 and YEATS2) and an activin/BMP signal component (ACVR2A) were recurrently altered. Furthermore, a novel FN1-ACVR2A fusion transcript was observed in both chondrosarcoma and osteochondromatosis cases. With the characteristic accumulative process of somatic changes as a background, molecular defects in chondrogenesis and aberrant epigenetic control are primarily causative of both benign and malignant cartilaginous tumors.

Original languageEnglish
Pages (from-to)1411-1420
Number of pages10
JournalGenome Research
Volume24
Issue number9
DOIs
Publication statusPublished - Sep 1 2014

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Unique mutation portraits and frequent COL2A1 gene alteration in chondrosarcoma'. Together they form a unique fingerprint.

  • Cite this

    Yoshida, A., Totoki, Y., Yoshida, A., Hosoda, F., Nakamura, H., Hama, N., Ogura, K., Fujiwara, T., Arai, Y., Toguchida, J., Tsuda, H., Miyano, S., Kawai, A., & Shibata, T. (2014). Unique mutation portraits and frequent COL2A1 gene alteration in chondrosarcoma. Genome Research, 24(9), 1411-1420. https://doi.org/10.1101/gr.160598.113