Unique genes induced by mechanical stress in periodontal ligament cells

Fumio Myokai, Masataka Oyama, Fusanori Nishimura, Taisuke Ohira, Tadashi Yamamoto, Hideo Arai, Shogo Takashiba, Yoji Murayama

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objectives: The aim of this study is to isolate mechanical stress-induced genes (MSGens) from human periodontal ligament (PDL) cells and to analyze profiles of the mRNA expression of these genes. Background: Differential expression of genes in PDL cells under physiological stress such as occlusal force is thought to be orchestrated not only for the remodeling of PDL itself but also for the repair and regeneration of periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress. Methods: The cDNA from mechanical stress-applied human PDL cells was subtracted against the cDNA from static control cells. The subtracted cDNA was amplified by polymerase chain reaction (PCR) and cloned for further analysis. Results: Among 68 independent clones isolated, 15 contained DNA fragments greater than 250 bp. Reverse Northern analysis revealed a marked induction of MSGen-15 and MSGen-28 mRNA expression in the mechanical stress-applied cells. However, little difference in the magnitude of expression for the other MSGens was detected between the stress-applied cells and the control cells. After nucleotide sequencing and the analysis of homology with known genes, five clones were identified; ribosomal protein S27 (MSGen-9), MRG 15 (MSGen-15), androgen-binding protein (MSGen-18), cathepsin H (MSGen-28), and cytochrome c (MSGen-47). Interestingly, it has been reported that MRG 15 is a novel transcription factor involved in the regulation of cell growth and senescence. The remaining 10 clones, classified into six sequence types, had no significant homology with any known genes. Conclusions: These results suggest that many known and unknown genes are expressed in response to mechanical stress in PDL cells, and that a transcription factor, MRG 15, may be responsible for molecular events in PDL cells under mechanical stress.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalJournal of Periodontal Research
Volume38
Issue number3
Publication statusPublished - Jun 2003

Fingerprint

Periodontal Ligament
Mechanical Stress
Genes
Complementary DNA
Clone Cells
Transcription Factors
Cathepsin H
Androgen-Binding Protein
Bite Force
Messenger RNA
Physiological Stress
Ribosomal Proteins
Cell Aging
Cytochromes c
Transcriptome
Regeneration
Nucleotides
Gene Expression
Polymerase Chain Reaction

Keywords

  • Differential gene expression
  • Mechanical stress
  • Periodontal ligament cells
  • Reverse Northern analysis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Myokai, F., Oyama, M., Nishimura, F., Ohira, T., Yamamoto, T., Arai, H., ... Murayama, Y. (2003). Unique genes induced by mechanical stress in periodontal ligament cells. Journal of Periodontal Research, 38(3), 255-261.

Unique genes induced by mechanical stress in periodontal ligament cells. / Myokai, Fumio; Oyama, Masataka; Nishimura, Fusanori; Ohira, Taisuke; Yamamoto, Tadashi; Arai, Hideo; Takashiba, Shogo; Murayama, Yoji.

In: Journal of Periodontal Research, Vol. 38, No. 3, 06.2003, p. 255-261.

Research output: Contribution to journalArticle

Myokai, F, Oyama, M, Nishimura, F, Ohira, T, Yamamoto, T, Arai, H, Takashiba, S & Murayama, Y 2003, 'Unique genes induced by mechanical stress in periodontal ligament cells', Journal of Periodontal Research, vol. 38, no. 3, pp. 255-261.
Myokai F, Oyama M, Nishimura F, Ohira T, Yamamoto T, Arai H et al. Unique genes induced by mechanical stress in periodontal ligament cells. Journal of Periodontal Research. 2003 Jun;38(3):255-261.
Myokai, Fumio ; Oyama, Masataka ; Nishimura, Fusanori ; Ohira, Taisuke ; Yamamoto, Tadashi ; Arai, Hideo ; Takashiba, Shogo ; Murayama, Yoji. / Unique genes induced by mechanical stress in periodontal ligament cells. In: Journal of Periodontal Research. 2003 ; Vol. 38, No. 3. pp. 255-261.
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AU - Yamamoto, Tadashi

AU - Arai, Hideo

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AU - Murayama, Yoji

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N2 - Objectives: The aim of this study is to isolate mechanical stress-induced genes (MSGens) from human periodontal ligament (PDL) cells and to analyze profiles of the mRNA expression of these genes. Background: Differential expression of genes in PDL cells under physiological stress such as occlusal force is thought to be orchestrated not only for the remodeling of PDL itself but also for the repair and regeneration of periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress. Methods: The cDNA from mechanical stress-applied human PDL cells was subtracted against the cDNA from static control cells. The subtracted cDNA was amplified by polymerase chain reaction (PCR) and cloned for further analysis. Results: Among 68 independent clones isolated, 15 contained DNA fragments greater than 250 bp. Reverse Northern analysis revealed a marked induction of MSGen-15 and MSGen-28 mRNA expression in the mechanical stress-applied cells. However, little difference in the magnitude of expression for the other MSGens was detected between the stress-applied cells and the control cells. After nucleotide sequencing and the analysis of homology with known genes, five clones were identified; ribosomal protein S27 (MSGen-9), MRG 15 (MSGen-15), androgen-binding protein (MSGen-18), cathepsin H (MSGen-28), and cytochrome c (MSGen-47). Interestingly, it has been reported that MRG 15 is a novel transcription factor involved in the regulation of cell growth and senescence. The remaining 10 clones, classified into six sequence types, had no significant homology with any known genes. Conclusions: These results suggest that many known and unknown genes are expressed in response to mechanical stress in PDL cells, and that a transcription factor, MRG 15, may be responsible for molecular events in PDL cells under mechanical stress.

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