Unidirectional binding of clostridial collagenase to triple helical substrates

Sagaya Theresa Leena Philominathan, Takaki Koide, Kentaro Hamada, Hiroyuki Yasui, Soenke Seifert, Osamu Matsushita, Joshua Sakon

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Histotoxic clostridia produce collagenases responsible for extensive tissue destruction in gas gangrene. The C-terminal collagen-binding domain (CBD) of these enzymes is the minimal segment required to bind to collagen fibril. Collagen binding efficiency of CBD is more pronounced in the presence of Ca2+. We have shown that CBD can be functional to anchor growth factors in local tissue. A 1H-15N HSQC NMR titration study with three different tropocollagen analogues ((POG)10)3, ((GPOG)7PRG)3, and (GPRG(POG)7C-carbamidomethyl)3, mapped a saddle-like binding cleft on CBD. NMR titrations with three nitroxide spin-labeled analogues of collagenous peptide, (PROXYL-G(POG)7PRG)3, (PROXYL-G(POG)7)3, and (GPRG(POG)7C-PROXYL)3 (where PROXYL represents 2,2,5,5-tetramethyl-L-pyrrolidinyloxy), unambiguously demonstrated unidirectional binding of CBD to the tropocollagen analogues. Small angle x-ray scattering data revealed that CBD binds closer to a terminus for each of the five different tropocollagen analogues, which in conjunction with NMR titration studies, implies a binding mode where CBD binds to the C terminus of the triple helix.

Original languageEnglish
Pages (from-to)10868-10876
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number16
DOIs
Publication statusPublished - Apr 17 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Philominathan, S. T. L., Koide, T., Hamada, K., Yasui, H., Seifert, S., Matsushita, O., & Sakon, J. (2009). Unidirectional binding of clostridial collagenase to triple helical substrates. Journal of Biological Chemistry, 284(16), 10868-10876. https://doi.org/10.1074/jbc.M807684200