Uni-axial cyclic stretch induces the activation of transcription factor nuclear factor kappaB in human fibroblast cells.

Hidefumi Inoh, Naoki Ishiguro, Shin Ichi Sawazaki, Hideki Amma, Motoi Miyazu, Hisashi Iwata, Masahiro Sokabe, Keiji Naruse

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The effect of uni-axial cyclic mechanical stretch on the activation of the transcription factor nuclear factor kappaB (NF-kappaB) was investigated in a human fibroblast cell line (TIG-1). In response to uni-axial cyclic stretch, NF-kappaB was found to be translocated into the nucleus. The NF-kappaB was first detectable 2 min after the onset of stretch and then peaked at 4 min and returned to the basal level within 10 min. To investigate whether NF-kappaB is activated following the translocation into the nucleus, we measured the luciferase activity in the cells transfected with pNF-kappaB-luciferase. The activity of luciferase increased 4 min after the initiation of cyclic stretch, peaked at 15 min (6.4-fold increase), and decreased gradually. We examined the involvement of the stretch-activated (SA) channel in the stretch-induced NF-kappaB activation. The application of Gd3+, a blocker of the SA channel, or the removal of extracellular Ca2+ inhibited both the translocation into the nucleus and the activation of NF-kB, which suggests that NF-kappaB is activated by uni-axial cyclic stretch via SA channel activation in human lung fibroblasts.

Original languageEnglish
Pages (from-to)405-407
Number of pages3
JournalFASEB Journal
Volume16
Issue number3
Publication statusPublished - 2002
Externally publishedYes

    Fingerprint

Cite this

Inoh, H., Ishiguro, N., Sawazaki, S. I., Amma, H., Miyazu, M., Iwata, H., Sokabe, M., & Naruse, K. (2002). Uni-axial cyclic stretch induces the activation of transcription factor nuclear factor kappaB in human fibroblast cells. FASEB Journal, 16(3), 405-407.