Uni-axial cyclic stretch induces c-src activation and translocation in human endothelial cells via SA channel activation

Keiji Naruse, Xaurui Sai, Nagao Yokoyama, Masahiro Sokabe

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The kinase activity of c-src increased and peaked at 15 min after an application of uni-axial cyclic stretch in HUVECs followed by a translocation of c-src to Triton-insoluble fraction. Suppression of c-src by an antisense S-oligodeoxynucleotide inhibited the stretch-induced tyrosine phosphorylation and morphological changes. The stretch-induced increase in c-src activity was inhibited by FK506, a specific inhibitor for calcineurin, by Gd3+, a blocker for stretch activated channels, and by the extracellular Ca2+ depletion suggesting the involvement of SA channels. These results strongly suggest c-src plays an important role in the downstream of SA channel activation followed by the morphological changes. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalFEBS Letters
Volume441
Issue number1
DOIs
Publication statusPublished - Dec 11 1998
Externally publishedYes

Fingerprint

Phosphorylation
Oligodeoxyribonucleotides
Endothelial cells
Tacrolimus
Tyrosine
Phosphotransferases
Endothelial Cells
Chemical activation
Calcineurin Inhibitors
CSK tyrosine-protein kinase

Keywords

  • Ca
  • Calcineurin
  • ECV304
  • FK506
  • Gd

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Uni-axial cyclic stretch induces c-src activation and translocation in human endothelial cells via SA channel activation. / Naruse, Keiji; Sai, Xaurui; Yokoyama, Nagao; Sokabe, Masahiro.

In: FEBS Letters, Vol. 441, No. 1, 11.12.1998, p. 111-115.

Research output: Contribution to journalArticle

Naruse, Keiji ; Sai, Xaurui ; Yokoyama, Nagao ; Sokabe, Masahiro. / Uni-axial cyclic stretch induces c-src activation and translocation in human endothelial cells via SA channel activation. In: FEBS Letters. 1998 ; Vol. 441, No. 1. pp. 111-115.
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