Uni-axial cyclic stretch induces c-src activation and translocation in human endothelial cells via SA channel activation

Keiji Naruse, Xaurui Sai, Nagao Yokoyama, Masahiro Sokabe

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The kinase activity of c-src increased and peaked at 15 min after an application of uni-axial cyclic stretch in HUVECs followed by a translocation of c-src to Triton-insoluble fraction. Suppression of c-src by an antisense S-oligodeoxynucleotide inhibited the stretch-induced tyrosine phosphorylation and morphological changes. The stretch-induced increase in c-src activity was inhibited by FK506, a specific inhibitor for calcineurin, by Gd3+, a blocker for stretch activated channels, and by the extracellular Ca2+ depletion suggesting the involvement of SA channels. These results strongly suggest c-src plays an important role in the downstream of SA channel activation followed by the morphological changes. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalFEBS Letters
Volume441
Issue number1
DOIs
Publication statusPublished - Dec 11 1998
Externally publishedYes

Keywords

  • Ca
  • Calcineurin
  • ECV304
  • FK506
  • Gd

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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