Abstract
Human glomerular basement membrane (GBM) is known to contain type IV collagen, heparan sulfate proteoglycan (HS-PG) and laminin in the extracellular matrix. in this study, ultrastructural distribution of these antigens was investigated in normal human glomeruli using the immunogold technique. Type IV collagen was present throughout the GBM, mainly on the lamina densa, and all over the mesangial region. HS-PG was present on the laminae rarae, predominantly on the lamina rara externa, and periphery of the mesangial region. Laminin was present throughout the GBM and peripheral region of the mesangial matrix. Our study demonstrated the preferential localization of each component in the GBM.
| Original language | English |
|---|---|
| Pages (from-to) | 933-939 |
| Number of pages | 7 |
| Journal | The Japanese Journal of Nephrology |
| Volume | 35 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
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Keywords
- glomerular basement membrane
- heparan sulfate proteoglycan
- immunoelectron microscopy
- laminin
- type IV collagen
ASJC Scopus subject areas
- Nephrology
Cite this
Ultrastructural localization of three major basement membrane components : Type iv collagen, heparan sulfate proteoglycan and laminin in the normal human GBM. / Haramoto, Toshinori; Makino, Hirofumi.
In: The Japanese Journal of Nephrology, Vol. 35, No. 8, 01.01.1993, p. 933-939.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ultrastructural localization of three major basement membrane components
T2 - Type iv collagen, heparan sulfate proteoglycan and laminin in the normal human GBM
AU - Haramoto, Toshinori
AU - Makino, Hirofumi
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Human glomerular basement membrane (GBM) is known to contain type IV collagen, heparan sulfate proteoglycan (HS-PG) and laminin in the extracellular matrix. in this study, ultrastructural distribution of these antigens was investigated in normal human glomeruli using the immunogold technique. Type IV collagen was present throughout the GBM, mainly on the lamina densa, and all over the mesangial region. HS-PG was present on the laminae rarae, predominantly on the lamina rara externa, and periphery of the mesangial region. Laminin was present throughout the GBM and peripheral region of the mesangial matrix. Our study demonstrated the preferential localization of each component in the GBM.
AB - Human glomerular basement membrane (GBM) is known to contain type IV collagen, heparan sulfate proteoglycan (HS-PG) and laminin in the extracellular matrix. in this study, ultrastructural distribution of these antigens was investigated in normal human glomeruli using the immunogold technique. Type IV collagen was present throughout the GBM, mainly on the lamina densa, and all over the mesangial region. HS-PG was present on the laminae rarae, predominantly on the lamina rara externa, and periphery of the mesangial region. Laminin was present throughout the GBM and peripheral region of the mesangial matrix. Our study demonstrated the preferential localization of each component in the GBM.
KW - glomerular basement membrane
KW - heparan sulfate proteoglycan
KW - immunoelectron microscopy
KW - laminin
KW - type IV collagen
UR - http://www.scopus.com/inward/record.url?scp=0027640550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027640550&partnerID=8YFLogxK
U2 - 10.14842/jpnjnephrol1959.35.933
DO - 10.14842/jpnjnephrol1959.35.933
M3 - Article
C2 - 8255003
AN - SCOPUS:0027640550
VL - 35
SP - 933
EP - 939
JO - Japanese Journal of Nephrology
JF - Japanese Journal of Nephrology
SN - 0385-2385
IS - 8
ER -