Ultrastructural localization of the three major basement membrane components - type IV collagen, heparan sulfate proteoglycan and laminin - in human membranous glomerulonephritis

Toshinori Haramoto, Hirofumi Makino, Shuji Ikeda, Jörgen Wieslander, Zensuze Ota

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Membranous glomerulonephritis (MN) is characterized by the presence of subepithelial immune complexes and thickening of the glomerular basement membrane (GBM). Immune complexes are recognized as subepithelial elec- tron-dense deposits (EDDs) by electron microscopy. We used immunogold electron microscopy to detect the GBM components - type IV collagen, hepa-ran sulfate proteoglycan (HS-PG) and laminin - in thickened GBM, and stud-ied the relationship between immune complexes and these GBM components. We demonstrate that the three major basement membrane components are distributed throughout the newly synthesized GBM. These findings suggest that type IV collagen, HS-PG, and laminin together comprise the spike-like structures and the newly synthesized GBM-like matrix in the thickened GBM of idiopathic MN and membranous lupus nephritis. The newly constructed matrix in the GBM appears to be composed of nearly normal GBM. In type IV collagen, the a1-chain was rarely present on the newly synthesized basement membrane in the lamina rara externa, while a3-chain was present on the sub-epithelial newly synthesized basement membrane. HS-PG was found within EDDs in membranous lupus nephritis. This suggests that anti-DNA antibody may cross-react with the HS-PG component of the GBM and thus form a subepithelial immune complex.

Original languageEnglish
Pages (from-to)30-36
Number of pages7
JournalAmerican Journal of Nephrology
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 1 1994

Keywords

  • Heparan sulfate proteoglycan
  • Immunoelectron microscopy
  • Laminin
  • Lupus nephritis
  • Membranous nephritis
  • Type IV collagen
  • α1-Chain (IV)
  • α3-Chain (IV)

ASJC Scopus subject areas

  • Nephrology

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