Ultrastructural localization of tartrate-resistant acid phosphatase activity in rat osteoblasts

Toshio Yamamoto, Hiroshi Nagai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ultrastructural localization of tartrate-resistant acid phosphatase (TRAP) activity in metaphyseal osteoblasts of young rats was detected and compared with the localization of tartrate-sensitive acid phosphatase (TSAP). TRAP activity was detected in lysosomes and Golgi complex, such as Golgi lamellae, vacuoles and vesicles. TSAP, detected as β-glycerophosphatase, was also located in Golgi lamellae, vacuoles and vesicles, and in lysosomes. Based on the ultrastructural features of TRAP-positive structures and the similarity of the distribution patterns of TRAP and TSAP, it is suggested that TRAP co exists with TSAP, and is located in lysosomes and secretory granules in osteoblasts.

Original languageEnglish
Pages (from-to)659-663
Number of pages5
JournalJournal of Electron Microscopy
Volume47
Issue number6
Publication statusPublished - 1998

Fingerprint

osteoblasts
phosphatases
Osteoblasts
Phosphatases
rats
Rats
Lysosomes
acids
Acids
lysosomes
Vacuoles
lamella
Secretory Vesicles
Golgi Apparatus
Tartrate-Resistant Acid Phosphatase
distribution (property)
tartrate-sensitive acid phosphatase
Catalyst activity

Keywords

  • Bone
  • Bone formation
  • Bone remodelling
  • Histochemistry
  • Osteoblast
  • Tartrate-resistant acid phosphatase

ASJC Scopus subject areas

  • Instrumentation

Cite this

Ultrastructural localization of tartrate-resistant acid phosphatase activity in rat osteoblasts. / Yamamoto, Toshio; Nagai, Hiroshi.

In: Journal of Electron Microscopy, Vol. 47, No. 6, 1998, p. 659-663.

Research output: Contribution to journalArticle

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