Ultrastructural localization of tartrate-resistant acid phosphatase activity in rat osteoblasts

Toshio Yamamoto, Hiroshi Nagai

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Ultrastructural localization of tartrate-resistant acid phosphatase (TRAP) activity in metaphyseal osteoblasts of young rats was detected and compared with the localization of tartrate-sensitive acid phosphatase (TSAP). TRAP activity was detected in lysosomes and Golgi complex, such as Golgi lamellae, vacuoles and vesicles. TSAP, detected as β-glycerophosphatase, was also located in Golgi lamellae, vacuoles and vesicles, and in lysosomes. Based on the ultrastructural features of TRAP-positive structures and the similarity of the distribution patterns of TRAP and TSAP, it is suggested that TRAP co exists with TSAP, and is located in lysosomes and secretory granules in osteoblasts.

Original languageEnglish
Pages (from-to)659-663
Number of pages5
JournalJournal of Electron Microscopy
Volume47
Issue number6
DOIs
Publication statusPublished - 1998

Keywords

  • Bone
  • Bone formation
  • Bone remodelling
  • Histochemistry
  • Osteoblast
  • Tartrate-resistant acid phosphatase

ASJC Scopus subject areas

  • Instrumentation

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