TY - JOUR
T1 - Ultrastructural localization of tartrate-resistant acid phosphatase activity in rat osteoblasts
AU - Yamamoto, Toshio
AU - Nagai, Hiroshi
PY - 1998
Y1 - 1998
N2 - Ultrastructural localization of tartrate-resistant acid phosphatase (TRAP) activity in metaphyseal osteoblasts of young rats was detected and compared with the localization of tartrate-sensitive acid phosphatase (TSAP). TRAP activity was detected in lysosomes and Golgi complex, such as Golgi lamellae, vacuoles and vesicles. TSAP, detected as β-glycerophosphatase, was also located in Golgi lamellae, vacuoles and vesicles, and in lysosomes. Based on the ultrastructural features of TRAP-positive structures and the similarity of the distribution patterns of TRAP and TSAP, it is suggested that TRAP co exists with TSAP, and is located in lysosomes and secretory granules in osteoblasts.
AB - Ultrastructural localization of tartrate-resistant acid phosphatase (TRAP) activity in metaphyseal osteoblasts of young rats was detected and compared with the localization of tartrate-sensitive acid phosphatase (TSAP). TRAP activity was detected in lysosomes and Golgi complex, such as Golgi lamellae, vacuoles and vesicles. TSAP, detected as β-glycerophosphatase, was also located in Golgi lamellae, vacuoles and vesicles, and in lysosomes. Based on the ultrastructural features of TRAP-positive structures and the similarity of the distribution patterns of TRAP and TSAP, it is suggested that TRAP co exists with TSAP, and is located in lysosomes and secretory granules in osteoblasts.
KW - Bone
KW - Bone formation
KW - Bone remodelling
KW - Histochemistry
KW - Osteoblast
KW - Tartrate-resistant acid phosphatase
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U2 - 10.1093/oxfordjournals.jmicro.a023639
DO - 10.1093/oxfordjournals.jmicro.a023639
M3 - Article
C2 - 9972546
AN - SCOPUS:0032421101
VL - 47
SP - 659
EP - 663
JO - Microscopy (Oxford, England)
JF - Microscopy (Oxford, England)
SN - 2050-5698
IS - 6
ER -