Ultrastructural localization of adenylate cyclase activity in chicken osteoclasts

O. Fukushima, T. Yamamoto, C. V. Gay

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Using lead citrate as a capture reagent and adenylate-(β,γ-methylene) diphosphate (AMP-PCP) as a substrate, we localized adenylate cyclase activity on the non-ruffled border plasma membrane of approximately half of the osteoclasts on trabecular bone surfaces in the tibial metaphyses of chickens fed a low (0.3%)-calcium diet. The enzyme was not detectable in osteoclasts when chickens were fed a normal calcium diet. Activity was observed on the entire plasma membrane of detached osteoclasts that were situated between osteoblasts on the bone surface and blood vessels in the marrow cavity. Detection of activity on detached osteoclasts required the presence of an activator, implying lower levels in these cells than in those with ruffled borders. Staining was greater on the lateral sides of osteoblasts and osteoclasts when they were in contact with each other. Reaction specificity was indicated by the demonstration of stimulation by forskolin, guanylate-(β,γ-methylene) diphosphate (GMP-PCP), dimethylsulfoxide, and NaF, inhibition by alloxan and 2',5'-dideoxyadenosine, and absence of activity when sections were incubated in substrate-free medium or when GMP-PCP replaced AMP-PCP as a substrate. The finding of adenylate cyclase in osteoclast plasma membrane provides structural evidence that the adenylate cyclase-cyclic AMP system has a role in regulation of osteoclast cell function. The low-calcium diet appears to have resulted in increased amounts of adenylate cyclase in osteoclasts.

Original languageEnglish
Pages (from-to)1207-1213
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume39
Issue number9
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Osteoclasts
Adenylyl Cyclases
Chickens
Diphosphates
Cell Membrane
Diet
Calcium
Osteoblasts
Alloxan
Colforsin
Dimethyl Sulfoxide
Citric Acid
Cyclic AMP
Blood Vessels
Bone Marrow
Staining and Labeling
Bone and Bones
Enzymes

Keywords

  • Adenylate cyclase histochemistry
  • Avian osteoclasts
  • Calcium-deficient diet

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Ultrastructural localization of adenylate cyclase activity in chicken osteoclasts. / Fukushima, O.; Yamamoto, T.; Gay, C. V.

In: Journal of Histochemistry and Cytochemistry, Vol. 39, No. 9, 1991, p. 1207-1213.

Research output: Contribution to journalArticle

Fukushima, O. ; Yamamoto, T. ; Gay, C. V. / Ultrastructural localization of adenylate cyclase activity in chicken osteoclasts. In: Journal of Histochemistry and Cytochemistry. 1991 ; Vol. 39, No. 9. pp. 1207-1213.
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