Ultrastructural binding site of pemphigus foliaceus autoantibodies: Comparison with pemphigus vulgaris

K. Iwatsuki, M. Takigawa, F. Jin, M. Yamada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We studied in vivo binding sites of pemphigus foliaceus (PF) autoantibodies by immuno-gold labelling technique, and compared them with those of pemphigus vulgaris (PV). In early acantholytic lesions of PF, the bound antibodies indicated by 5 nm protein A-colloidal gold particles were observed on the surface of keratinocytes, with particular affinity for desmosomes and separated attachment plaques. Nondesmosomal cell surfaces were sparsely labeled with the gold particles. A similar binding pattern was seen in the epidermal sheets obtained from a PV patient utilizing the Nikolsky phenomenon. These findings indicate that both PF and PV antigen-antibody complexes are densely located on the desmosomal areas in early pemphigus lesions, suggesting the pathogenic importance of functional impairment of desmosomes by the autoantibodies.

Original languageEnglish
Pages (from-to)160-163
Number of pages4
JournalJournal of Cutaneous Pathology
Volume18
Issue number3
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Pemphigus
Autoantibodies
Binding Sites
Desmosomes
Gold
Gold Colloid
Staphylococcal Protein A
Antigen-Antibody Complex
Keratinocytes
Antibodies

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine

Cite this

Ultrastructural binding site of pemphigus foliaceus autoantibodies : Comparison with pemphigus vulgaris. / Iwatsuki, K.; Takigawa, M.; Jin, F.; Yamada, M.

In: Journal of Cutaneous Pathology, Vol. 18, No. 3, 1991, p. 160-163.

Research output: Contribution to journalArticle

Iwatsuki, K. ; Takigawa, M. ; Jin, F. ; Yamada, M. / Ultrastructural binding site of pemphigus foliaceus autoantibodies : Comparison with pemphigus vulgaris. In: Journal of Cutaneous Pathology. 1991 ; Vol. 18, No. 3. pp. 160-163.
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