Ultrastructural analysis of an enterolith composed of Deoxycholic acid

Masaya Iwamuro, Yuichi Miyashima, Takahiro Yoshioka, Toshihiro Murata, Yoshio Miyabe, Yoshinari Kawai, Haruo Urata, Hidenori Shiraha, Hiroyuki Okada, Kazuhide Yamamoto

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2 Citations (Scopus)

Abstract

A 67-year-old Japanese man underwent enterotomy because of enterolith ileus. Component analysis by infrared spectroscopy revealed that the enterolith was composed of a high concentration of deoxycholic acid. We further analyzed and compared the ultrastructure of the enterolith and a commercially available powdered form of deoxycholic acid by means of scanning electron microscopy and energy dispersive X-ray spectroscopy. Energy dispersive X-ray spectroscopy analysis revealed that the ratios of carbon and oxygen in the enterolith were equal to those in the deoxycholic acid powder. Scanning electron microscopy analysis showed rectangular prism-shaped particles on the surface of the enterolith. This structure was similar to that of the deoxycholic acid powder. The surgically removed enterolith had a twisted and coiled appearance. Possible mechanisms underlying the formation of this unique form are discussed.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalActa medica Okayama
Volume68
Issue number6
Publication statusPublished - Jan 1 2014

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Keywords

  • Deoxycholic acid
  • Energy dispersive X-ray spectroscopy
  • Enterolith
  • Infrared spectroscopy
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Iwamuro, M., Miyashima, Y., Yoshioka, T., Murata, T., Miyabe, Y., Kawai, Y., Urata, H., Shiraha, H., Okada, H., & Yamamoto, K. (2014). Ultrastructural analysis of an enterolith composed of Deoxycholic acid. Acta medica Okayama, 68(6), 369-374.