TY - JOUR
T1 - Ultrasound-guided non-surgical embryo collection in the common marmoset
AU - Ishibashi, Hidetoshi
AU - Motohashi, Hideyuki H.
AU - Kumon, Mami
AU - Yamamoto, Kazuhiro
AU - Okada, Hironori
AU - Okada, Takashi
AU - Seki, Kazuhiko
N1 - Funding Information:
This work was supported by The Research Grant ( 20-10 ) for Nervous and Mental Disorders from the Ministry of Health, Labor and Welfare , and an Intramural Research Grant ( 23-9 ) for Neurological and Psychiatric Disorders from the NCNP . We are also thankful for the generous support and encouragement from Drs. Katsuki Nakamura, Keiji Wada, Shin’ichi Takeda, and Shinichi Kohsaka.
PY - 2013/6
Y1 - 2013/6
N2 - Experimental primate embryology has been hampered by limited access to embryos. In addition to surgical techniques, the less stressful non-surgical technique of uterine flushing has been developed but has had only limitedly used in recovering pre-implantation embryos from marmoset monkeys. In this study, we introduce the use of ultrasonography during marmoset non-surgical uterine flushing to make the cannulation easier, to further reduce stress, and to ensure thorough uterine flushing. We were able to cannulate in 99% of the transcervical cannulation attempts, repeat the flushing up to 17 times with the same animal, and recover up to 90% of the ovulation products. We also found that 8-cell or earlier stage embryos could be frequently obtained by non-surgical uterine flushing at 4 or 5 days after ovulation. The easiness and effectiveness of this novel ultrasound-guided technique will enable more research groups to study marmoset embryology and facilitate progress in this field.
AB - Experimental primate embryology has been hampered by limited access to embryos. In addition to surgical techniques, the less stressful non-surgical technique of uterine flushing has been developed but has had only limitedly used in recovering pre-implantation embryos from marmoset monkeys. In this study, we introduce the use of ultrasonography during marmoset non-surgical uterine flushing to make the cannulation easier, to further reduce stress, and to ensure thorough uterine flushing. We were able to cannulate in 99% of the transcervical cannulation attempts, repeat the flushing up to 17 times with the same animal, and recover up to 90% of the ovulation products. We also found that 8-cell or earlier stage embryos could be frequently obtained by non-surgical uterine flushing at 4 or 5 days after ovulation. The easiness and effectiveness of this novel ultrasound-guided technique will enable more research groups to study marmoset embryology and facilitate progress in this field.
KW - Embryo collection
KW - Flushing
KW - Nonhuman primate
KW - Repeatability
KW - Transcervical
KW - Ultrasonography
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U2 - 10.1016/j.repbio.2013.02.002
DO - 10.1016/j.repbio.2013.02.002
M3 - Article
C2 - 23719119
AN - SCOPUS:84883793192
VL - 13
SP - 139
EP - 144
JO - Reproductive biology
JF - Reproductive biology
SN - 1642-431X
IS - 2
ER -