Ultrasound-guided non-surgical embryo collection in the common marmoset

Hidetoshi Ishibashi, Hideyuki H. Motohashi, Mami Kumon, Kazuhiro Yamamoto, Hironori Okada, Takashi Okada, Kazuhiko Seki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Experimental primate embryology has been hampered by limited access to embryos. In addition to surgical techniques, the less stressful non-surgical technique of uterine flushing has been developed but has had only limitedly used in recovering pre-implantation embryos from marmoset monkeys. In this study, we introduce the use of ultrasonography during marmoset non-surgical uterine flushing to make the cannulation easier, to further reduce stress, and to ensure thorough uterine flushing. We were able to cannulate in 99% of the transcervical cannulation attempts, repeat the flushing up to 17 times with the same animal, and recover up to 90% of the ovulation products. We also found that 8-cell or earlier stage embryos could be frequently obtained by non-surgical uterine flushing at 4 or 5 days after ovulation. The easiness and effectiveness of this novel ultrasound-guided technique will enable more research groups to study marmoset embryology and facilitate progress in this field.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalReproductive biology
Volume13
Issue number2
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

Fingerprint

Callithrix
Callithrix jacchus
Callitrichidae
embryo (animal)
embryology
Embryology
Embryonic Structures
Ovulation
Catheterization
ovulation
embryo implantation
ultrasonography
Primates
Haplorhini
monkeys
Ultrasonography
surgery
methodology
Research
animals

Keywords

  • Embryo collection
  • Flushing
  • Nonhuman primate
  • Repeatability
  • Transcervical
  • Ultrasonography

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology
  • Endocrinology

Cite this

Ishibashi, H., Motohashi, H. H., Kumon, M., Yamamoto, K., Okada, H., Okada, T., & Seki, K. (2013). Ultrasound-guided non-surgical embryo collection in the common marmoset. Reproductive biology, 13(2), 139-144. https://doi.org/10.1016/j.repbio.2013.02.002

Ultrasound-guided non-surgical embryo collection in the common marmoset. / Ishibashi, Hidetoshi; Motohashi, Hideyuki H.; Kumon, Mami; Yamamoto, Kazuhiro; Okada, Hironori; Okada, Takashi; Seki, Kazuhiko.

In: Reproductive biology, Vol. 13, No. 2, 06.2013, p. 139-144.

Research output: Contribution to journalArticle

Ishibashi, H, Motohashi, HH, Kumon, M, Yamamoto, K, Okada, H, Okada, T & Seki, K 2013, 'Ultrasound-guided non-surgical embryo collection in the common marmoset', Reproductive biology, vol. 13, no. 2, pp. 139-144. https://doi.org/10.1016/j.repbio.2013.02.002
Ishibashi H, Motohashi HH, Kumon M, Yamamoto K, Okada H, Okada T et al. Ultrasound-guided non-surgical embryo collection in the common marmoset. Reproductive biology. 2013 Jun;13(2):139-144. https://doi.org/10.1016/j.repbio.2013.02.002
Ishibashi, Hidetoshi ; Motohashi, Hideyuki H. ; Kumon, Mami ; Yamamoto, Kazuhiro ; Okada, Hironori ; Okada, Takashi ; Seki, Kazuhiko. / Ultrasound-guided non-surgical embryo collection in the common marmoset. In: Reproductive biology. 2013 ; Vol. 13, No. 2. pp. 139-144.
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