Abstract
Experimental primate embryology has been hampered by limited access to embryos. In addition to surgical techniques, the less stressful non-surgical technique of uterine flushing has been developed but has had only limitedly used in recovering pre-implantation embryos from marmoset monkeys. In this study, we introduce the use of ultrasonography during marmoset non-surgical uterine flushing to make the cannulation easier, to further reduce stress, and to ensure thorough uterine flushing. We were able to cannulate in 99% of the transcervical cannulation attempts, repeat the flushing up to 17 times with the same animal, and recover up to 90% of the ovulation products. We also found that 8-cell or earlier stage embryos could be frequently obtained by non-surgical uterine flushing at 4 or 5 days after ovulation. The easiness and effectiveness of this novel ultrasound-guided technique will enable more research groups to study marmoset embryology and facilitate progress in this field.
| Original language | English |
|---|---|
| Pages (from-to) | 139-144 |
| Number of pages | 6 |
| Journal | Reproductive biology |
| Volume | 13 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 2013 |
| Externally published | Yes |
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Keywords
- Embryo collection
- Flushing
- Nonhuman primate
- Repeatability
- Transcervical
- Ultrasonography
ASJC Scopus subject areas
- Animal Science and Zoology
- Developmental Biology
- Endocrinology
Cite this
Ultrasound-guided non-surgical embryo collection in the common marmoset. / Ishibashi, Hidetoshi; Motohashi, Hideyuki H.; Kumon, Mami; Yamamoto, Kazuhiro; Okada, Hironori; Okada, Takashi; Seki, Kazuhiko.
In: Reproductive biology, Vol. 13, No. 2, 06.2013, p. 139-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ultrasound-guided non-surgical embryo collection in the common marmoset
AU - Ishibashi, Hidetoshi
AU - Motohashi, Hideyuki H.
AU - Kumon, Mami
AU - Yamamoto, Kazuhiro
AU - Okada, Hironori
AU - Okada, Takashi
AU - Seki, Kazuhiko
PY - 2013/6
Y1 - 2013/6
N2 - Experimental primate embryology has been hampered by limited access to embryos. In addition to surgical techniques, the less stressful non-surgical technique of uterine flushing has been developed but has had only limitedly used in recovering pre-implantation embryos from marmoset monkeys. In this study, we introduce the use of ultrasonography during marmoset non-surgical uterine flushing to make the cannulation easier, to further reduce stress, and to ensure thorough uterine flushing. We were able to cannulate in 99% of the transcervical cannulation attempts, repeat the flushing up to 17 times with the same animal, and recover up to 90% of the ovulation products. We also found that 8-cell or earlier stage embryos could be frequently obtained by non-surgical uterine flushing at 4 or 5 days after ovulation. The easiness and effectiveness of this novel ultrasound-guided technique will enable more research groups to study marmoset embryology and facilitate progress in this field.
AB - Experimental primate embryology has been hampered by limited access to embryos. In addition to surgical techniques, the less stressful non-surgical technique of uterine flushing has been developed but has had only limitedly used in recovering pre-implantation embryos from marmoset monkeys. In this study, we introduce the use of ultrasonography during marmoset non-surgical uterine flushing to make the cannulation easier, to further reduce stress, and to ensure thorough uterine flushing. We were able to cannulate in 99% of the transcervical cannulation attempts, repeat the flushing up to 17 times with the same animal, and recover up to 90% of the ovulation products. We also found that 8-cell or earlier stage embryos could be frequently obtained by non-surgical uterine flushing at 4 or 5 days after ovulation. The easiness and effectiveness of this novel ultrasound-guided technique will enable more research groups to study marmoset embryology and facilitate progress in this field.
KW - Embryo collection
KW - Flushing
KW - Nonhuman primate
KW - Repeatability
KW - Transcervical
KW - Ultrasonography
UR - http://www.scopus.com/inward/record.url?scp=84883793192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883793192&partnerID=8YFLogxK
U2 - 10.1016/j.repbio.2013.02.002
DO - 10.1016/j.repbio.2013.02.002
M3 - Article
C2 - 23719119
AN - SCOPUS:84883793192
VL - 13
SP - 139
EP - 144
JO - Reproductive biology
JF - Reproductive biology
SN - 1642-431X
IS - 2
ER -