Ultrasound-guided non-surgical embryo collection in the common marmoset

Hidetoshi Ishibashi, Hideyuki H. Motohashi, Mami Kumon, Kazuhiro Yamamoto, Hironori Okada, Takashi Okada, Kazuhiko Seki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Experimental primate embryology has been hampered by limited access to embryos. In addition to surgical techniques, the less stressful non-surgical technique of uterine flushing has been developed but has had only limitedly used in recovering pre-implantation embryos from marmoset monkeys. In this study, we introduce the use of ultrasonography during marmoset non-surgical uterine flushing to make the cannulation easier, to further reduce stress, and to ensure thorough uterine flushing. We were able to cannulate in 99% of the transcervical cannulation attempts, repeat the flushing up to 17 times with the same animal, and recover up to 90% of the ovulation products. We also found that 8-cell or earlier stage embryos could be frequently obtained by non-surgical uterine flushing at 4 or 5 days after ovulation. The easiness and effectiveness of this novel ultrasound-guided technique will enable more research groups to study marmoset embryology and facilitate progress in this field.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalReproductive Biology
Volume13
Issue number2
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Embryo collection
  • Flushing
  • Nonhuman primate
  • Repeatability
  • Transcervical
  • Ultrasonography

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology
  • Developmental Biology

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    Ishibashi, H., Motohashi, H. H., Kumon, M., Yamamoto, K., Okada, H., Okada, T., & Seki, K. (2013). Ultrasound-guided non-surgical embryo collection in the common marmoset. Reproductive Biology, 13(2), 139-144. https://doi.org/10.1016/j.repbio.2013.02.002