Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate

Nanako Sumi, Shota Nagahiro, Eiji Nakata, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journalArticlepeer-review

Abstract

Tat-U1A-rose bengal conjugate (TatU1A-RB) was prepared as an ultrasound-sensitive RNA carrier molecule. This molecule consists of Tat cell-penetrating peptide, U1A RNA-binding protein, and rose bengal as a sonosensitizer. We demonstrated that TatU1A-RB delivered RNA via the endocytosis pathway, which was followed by ultrasound-dependent endosomal escape and cytosolic dispersion of the RNA. A short hairpin RNA (shRNA) delivered by TatU1A-RB mediated RNA interference (RNAi) ultrasound-dependently. Even by ultrasound irradiation through blood cells, RNAi could be induced with TatU1A-RB and the shRNA. This ultrasound-dependent cytosolic RNA delivery method will serve as the basis for a new approach to nucleic acid therapeutics.

Original languageEnglish
Article number128767
JournalBioorganic and Medicinal Chemistry Letters
Volume68
DOIs
Publication statusPublished - Jul 15 2022

Keywords

  • RNA delivery
  • RNAi
  • Rose Bengal
  • Sonosensitizer
  • Ultrasound

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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