Ultrafiltration of priming blood before cardiopulmonary bypass attenuates inflammatory response and maintains cardiopulmonary function in neonatal piglets

Shinya Ugaki, Osami Honjo, Yasuhiro Kotani, Mahito Nakakura, Takuma Douguchi, Yu Oshima, Ko Yoshizumi, Shingo Kasahara, Shunji Sano

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Blood priming is necessary for cardiopulmonary bypass (CPB) in neonates to avoid excessive hemodilution; however, transfusion-related inflammation affects postCPB outcomes in neonatal open-heart surgery. We hypothesized that ultrafiltration of priming blood before CPB may reduce inflammatory mediators in priming blood and postCPB inflammatory responses, thereby improving cardiopulmonary function. Twelve 1-week-old piglets (3.5 ± 0.2 kg) were divided into two groups. Group U (n = 6) employed the priming blood ultrafiltrated before CPB, but group N (n = 6) used the nonultrafiltrated blood. Cardiopulmonary bypass was performed for 2 hours and then modified ultrafiltration (MUF) was conducted. Data were acquired before CPB and after MUF. The values of K, serotonin, and IL-8 in priming blood was significantly decreased after ultrafiltration (8.2 ± 2.6 vs. 4.2 ± 0.8 mEq/L, p <0.01, 234 ± 96 vs. 74 ± 42 ng/ml, p <0.01, 78.4 ± 5.1 vs. 64.5 ± 59.1 pg/ml, p <0.05). Group U after MUF had lower thrombin-antithrombin complex levels (23.9 ± 5.1 vs. 33.7 ± 4.6 ng/ml, p <0.01) and lower IL-8 levels in airway fluid (925 ± 710 vs. 2495 ± 1207 pg/ml, p <0.05) than group N. Cardiac output and arterial PO2 after MUF in group U were also higher (1.13 ± 0.21 vs. 0.69 ± 0.22, p <0.01, 340 ± 190 vs. 149 ± 84 mm Hg, p <0.05). The ultrafiltration of blood priming before CPB attenuated activation of the coagulation pathway and inflammatory responses and preserved cardiopulmonary function in neonatal piglets.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalASAIO Journal
Volume55
Issue number3
DOIs
Publication statusPublished - May 2009

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Ultrafiltration
Cardiopulmonary Bypass
Blood
Interleukin-8
Hemodilution
Coagulation
Cardiac Output
Surgery
Thoracic Surgery
Serotonin
Chemical activation
Inflammation
Fluids

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

Cite this

Ultrafiltration of priming blood before cardiopulmonary bypass attenuates inflammatory response and maintains cardiopulmonary function in neonatal piglets. / Ugaki, Shinya; Honjo, Osami; Kotani, Yasuhiro; Nakakura, Mahito; Douguchi, Takuma; Oshima, Yu; Yoshizumi, Ko; Kasahara, Shingo; Sano, Shunji.

In: ASAIO Journal, Vol. 55, No. 3, 05.2009, p. 291-295.

Research output: Contribution to journalArticle

Ugaki, Shinya ; Honjo, Osami ; Kotani, Yasuhiro ; Nakakura, Mahito ; Douguchi, Takuma ; Oshima, Yu ; Yoshizumi, Ko ; Kasahara, Shingo ; Sano, Shunji. / Ultrafiltration of priming blood before cardiopulmonary bypass attenuates inflammatory response and maintains cardiopulmonary function in neonatal piglets. In: ASAIO Journal. 2009 ; Vol. 55, No. 3. pp. 291-295.
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