Ultrafast Excitation Energy Dynamics in a Diatom Photosystem I-Antenna Complex: A Femtosecond Fluorescence Upconversion Study

Ryo Nagao, Kohei Kagatani, Yoshifumi Ueno, Jian-Ren Shen, Seiji Akimoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fucoxanthin chlorophyll (Chl) a/c-binding proteins (FCPs) are unique light-harvesting antennas in diatoms. Recent time-resolved fluorescence analysis of photosystem I with FCP associated (PSI-FCPI) has mainly shown excitation energy transfer among Chls a from FCPI to PSI in tens of picoseconds. However, it remains unclear how each pigment, especially carotenoids and Chl c, in the FCPI is functionally related to the energy transfer in a femtosecond time range. Here, we reveal ultrafast excitation energy transfer mechanism in the PSI-FCPI preparations isolated from a diatom, Chaetoceros gracilis, by means of femtosecond time-resolved fluorescence spectroscopy with an upconversion system. Compared with the fluorescence lifetime components of PSI core-like complexes, the energy transfer of Chl c →Chl a in the FCPI was observed within hundreds of femtoseconds, and the energy in the FCPI was transferred to PSI in ∼2 ps. The comparative fluorescence analyses provide physical insights into the energy transfer machinery within FCPI and from FCPI to PSI.

Original languageEnglish
Pages (from-to)2673-2678
Number of pages6
JournalJournal of Physical Chemistry B
Volume123
Issue number12
DOIs
Publication statusPublished - Mar 28 2019

Fingerprint

Photosystem I Protein Complex
Diatoms
Excitation energy
Energy Transfer
algae
Energy transfer
chlorophylls
Chlorophyll
antennas
Fluorescence
energy transfer
Antennas
fluorescence
excitation
energy
Chlorophyll Binding Proteins
carotenoids
Fluorescence Spectrometry
machinery
Fluorescence spectroscopy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Ultrafast Excitation Energy Dynamics in a Diatom Photosystem I-Antenna Complex : A Femtosecond Fluorescence Upconversion Study. / Nagao, Ryo; Kagatani, Kohei; Ueno, Yoshifumi; Shen, Jian-Ren; Akimoto, Seiji.

In: Journal of Physical Chemistry B, Vol. 123, No. 12, 28.03.2019, p. 2673-2678.

Research output: Contribution to journalArticle

@article{75f2eee1d3b247179fcfeb1ed495b723,
title = "Ultrafast Excitation Energy Dynamics in a Diatom Photosystem I-Antenna Complex: A Femtosecond Fluorescence Upconversion Study",
abstract = "Fucoxanthin chlorophyll (Chl) a/c-binding proteins (FCPs) are unique light-harvesting antennas in diatoms. Recent time-resolved fluorescence analysis of photosystem I with FCP associated (PSI-FCPI) has mainly shown excitation energy transfer among Chls a from FCPI to PSI in tens of picoseconds. However, it remains unclear how each pigment, especially carotenoids and Chl c, in the FCPI is functionally related to the energy transfer in a femtosecond time range. Here, we reveal ultrafast excitation energy transfer mechanism in the PSI-FCPI preparations isolated from a diatom, Chaetoceros gracilis, by means of femtosecond time-resolved fluorescence spectroscopy with an upconversion system. Compared with the fluorescence lifetime components of PSI core-like complexes, the energy transfer of Chl c →Chl a in the FCPI was observed within hundreds of femtoseconds, and the energy in the FCPI was transferred to PSI in ∼2 ps. The comparative fluorescence analyses provide physical insights into the energy transfer machinery within FCPI and from FCPI to PSI.",
author = "Ryo Nagao and Kohei Kagatani and Yoshifumi Ueno and Jian-Ren Shen and Seiji Akimoto",
year = "2019",
month = "3",
day = "28",
doi = "10.1021/acs.jpcb.8b12086",
language = "English",
volume = "123",
pages = "2673--2678",
journal = "Journal of Physical Chemistry B Materials",
issn = "1520-6106",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Ultrafast Excitation Energy Dynamics in a Diatom Photosystem I-Antenna Complex

T2 - A Femtosecond Fluorescence Upconversion Study

AU - Nagao, Ryo

AU - Kagatani, Kohei

AU - Ueno, Yoshifumi

AU - Shen, Jian-Ren

AU - Akimoto, Seiji

PY - 2019/3/28

Y1 - 2019/3/28

N2 - Fucoxanthin chlorophyll (Chl) a/c-binding proteins (FCPs) are unique light-harvesting antennas in diatoms. Recent time-resolved fluorescence analysis of photosystem I with FCP associated (PSI-FCPI) has mainly shown excitation energy transfer among Chls a from FCPI to PSI in tens of picoseconds. However, it remains unclear how each pigment, especially carotenoids and Chl c, in the FCPI is functionally related to the energy transfer in a femtosecond time range. Here, we reveal ultrafast excitation energy transfer mechanism in the PSI-FCPI preparations isolated from a diatom, Chaetoceros gracilis, by means of femtosecond time-resolved fluorescence spectroscopy with an upconversion system. Compared with the fluorescence lifetime components of PSI core-like complexes, the energy transfer of Chl c →Chl a in the FCPI was observed within hundreds of femtoseconds, and the energy in the FCPI was transferred to PSI in ∼2 ps. The comparative fluorescence analyses provide physical insights into the energy transfer machinery within FCPI and from FCPI to PSI.

AB - Fucoxanthin chlorophyll (Chl) a/c-binding proteins (FCPs) are unique light-harvesting antennas in diatoms. Recent time-resolved fluorescence analysis of photosystem I with FCP associated (PSI-FCPI) has mainly shown excitation energy transfer among Chls a from FCPI to PSI in tens of picoseconds. However, it remains unclear how each pigment, especially carotenoids and Chl c, in the FCPI is functionally related to the energy transfer in a femtosecond time range. Here, we reveal ultrafast excitation energy transfer mechanism in the PSI-FCPI preparations isolated from a diatom, Chaetoceros gracilis, by means of femtosecond time-resolved fluorescence spectroscopy with an upconversion system. Compared with the fluorescence lifetime components of PSI core-like complexes, the energy transfer of Chl c →Chl a in the FCPI was observed within hundreds of femtoseconds, and the energy in the FCPI was transferred to PSI in ∼2 ps. The comparative fluorescence analyses provide physical insights into the energy transfer machinery within FCPI and from FCPI to PSI.

UR - http://www.scopus.com/inward/record.url?scp=85063134486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063134486&partnerID=8YFLogxK

U2 - 10.1021/acs.jpcb.8b12086

DO - 10.1021/acs.jpcb.8b12086

M3 - Article

C2 - 30807170

AN - SCOPUS:85063134486

VL - 123

SP - 2673

EP - 2678

JO - Journal of Physical Chemistry B Materials

JF - Journal of Physical Chemistry B Materials

SN - 1520-6106

IS - 12

ER -