Ubiquitination regulates MHC class II-peptide complex retention and degradation in dendritic cells

Even Walseng, Kazuyuki Furuta, Berta Bosch, Karis A. Weih, Yohei Matsuki, Oddmund Bakke, Satoshi Ishido, Paul A. Roche

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The expression and turnover of MHC class II-peptide complexes (pMHC-II) on the surface of dendritic cells (DCs) is essential for their ability to activate CD4 T cells efficiently. The half-life of surface pMHC-II is significantly greater in activated (mature) DCs than in resting (immature) DCs, but the molecular mechanism leading to this difference remains unknown. We now show that ubiquitination of pMHC-II by the E3 ubiquitin ligase membrane-associated RING-CH 1 (March-I) regulates surface expression, intracellular distribution, and survival of pMHC-II in DCs. DCs isolated from March- I-KO mice express very high levels of pMHC-II on the plasma membrane even before DC activation. Although ubiquitination does not affect the kinetics of pMHC-II endocytosis from the surface of DCs, the survival of pMHC-II is enhanced in DCs obtained from March-I- deficient and MHC-II ubiquitination-mutant mice. Using pMHC-II- specific mAb, we show that immature DCs generate large amounts of pMHC-II that are remarkably stable under conditions in which pMHC-II ubiquitination is blocked. Thus, the cellular distribution and stability of surface pMHC-II in DCs is regulated by ubiquitindependent degradation of internalized pMHC-II.

Original languageEnglish
Pages (from-to)20465-20470
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number47
DOIs
Publication statusPublished - Nov 23 2010
Externally publishedYes

ASJC Scopus subject areas

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