TY - JOUR
T1 - Tyrosine kinase-type receptor ErbB4 in chondrocytes
T2 - Interaction with connective tissue growth factor and distribution in cartilage
AU - Nawachi, Kumiko
AU - Inoue, Miho
AU - Kubota, Satoshi
AU - Nishida, Takashi
AU - Yosimichi, Gen
AU - Nakanishi, Tohru
AU - Kanyama, Manabu
AU - Kuboki, Takuo
AU - Yatani, Hirofumi
AU - Yamaai, Tomoichiro
AU - Takigawa, Masaharu
N1 - Funding Information:
The authors wish to thank Dr. Carmen Birchmeier (MDC-Berlin) for a gift of a cDNA of ErbB4; Drs. Takanori Eguchi, Seiji Kondo, and Norifumi H. Moritani for their helpful suggestions and discussions; Ms. Kazumi Ohyama for technical assistance; and Ms. Yuki Nonami for secretarial assistance. This work was supported in part by grants-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture of Japan (S.K., T.N., M.T.), a grant-in aid for specific diseases from the Ministry of Public Health and Welfare of Japan (M.T.), and by grants from the Nakatomi Health Science Foundation (S.K.), Sumitomo Foundation (M.T.), and Ryobi-teien Memorial Foundation (S.K.).
PY - 2002/9/25
Y1 - 2002/9/25
N2 - In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.
AB - In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.
KW - Cartilage
KW - Chondrocyte
KW - Connective tissue growth factor
KW - ErbB4
KW - Receptor
UR - http://www.scopus.com/inward/record.url?scp=18644362231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18644362231&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(02)03263-5
DO - 10.1016/S0014-5793(02)03263-5
M3 - Article
C2 - 12297288
AN - SCOPUS:18644362231
SN - 0014-5793
VL - 528
SP - 109
EP - 113
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -