Typing of Y chromosome single nucleotide polymorphisms in a Japanese population by a multiplexed single nucleotide primer extension reaction

Sachiyo Inagaki, Yuji Yamamoto, Yusuke Doi, Tomoyo Takata, Takaki Ishikawa, Kei Yoshitome, Satoru Miyaishi, Hideo Ishizu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have developed a new method for typing single nucleotide polymorphisms (SNPs) on the human Y chromosome based on a multiplexed single nucleotide primer extension. This method has the advantage that several SNPs are typed rapidly and simultaneously. We examined 15 different SNP loci on Y chromosome, M9, M105, M122, M125, M128, M130, SRY465, IMS-JST006241, IMS-JST006841, IMS-JST002611, IMS-JST003305, IMS-JST008425, IMS-JST021354, IMS-JST021355 and IMS-JST055457, in 159 Japanese males. From the typing results of these 15 loci, we found 13 haplotypes. Gene diversity for each locus ranged from 0.025 to 0.486 and the haplotype diversity was estimated to be 0.838. This method could be readily applied for personal identification and paternity testing.

Original languageEnglish
Pages (from-to)202-206
Number of pages5
JournalLegal Medicine
Volume4
Issue number3
DOIs
Publication statusPublished - Sep 2002

Keywords

  • Haplotype diversity
  • Single nucleotide polymorphisms
  • Single nucleotide primer extension
  • Y chromosome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

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