Abstract
We have developed a new method for typing single nucleotide polymorphisms (SNPs) on the human Y chromosome based on a multiplexed single nucleotide primer extension. This method has the advantage that several SNPs are typed rapidly and simultaneously. We examined 15 different SNP loci on Y chromosome, M9, M105, M122, M125, M128, M130, SRY465, IMS-JST006241, IMS-JST006841, IMS-JST002611, IMS-JST003305, IMS-JST008425, IMS-JST021354, IMS-JST021355 and IMS-JST055457, in 159 Japanese males. From the typing results of these 15 loci, we found 13 haplotypes. Gene diversity for each locus ranged from 0.025 to 0.486 and the haplotype diversity was estimated to be 0.838. This method could be readily applied for personal identification and paternity testing.
| Original language | English |
|---|---|
| Pages (from-to) | 202-206 |
| Number of pages | 5 |
| Journal | Legal Medicine |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2002 |
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Keywords
- Haplotype diversity
- Single nucleotide polymorphisms
- Single nucleotide primer extension
- Y chromosome
ASJC Scopus subject areas
- Genetics
- Pathology and Forensic Medicine
- Psychiatry and Mental health
- Public Health, Environmental and Occupational Health
Cite this
Typing of Y chromosome single nucleotide polymorphisms in a Japanese population by a multiplexed single nucleotide primer extension reaction. / Inagaki, Sachiyo; Yamamoto, Yuji; Doi, Yusuke; Takata, Tomoyo; Ishikawa, Takaki; Yoshitome, Kei; Miyaishi, Satoru; Ishizu, Hideo.
In: Legal Medicine, Vol. 4, No. 3, 09.2002, p. 202-206.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Typing of Y chromosome single nucleotide polymorphisms in a Japanese population by a multiplexed single nucleotide primer extension reaction
AU - Inagaki, Sachiyo
AU - Yamamoto, Yuji
AU - Doi, Yusuke
AU - Takata, Tomoyo
AU - Ishikawa, Takaki
AU - Yoshitome, Kei
AU - Miyaishi, Satoru
AU - Ishizu, Hideo
PY - 2002/9
Y1 - 2002/9
N2 - We have developed a new method for typing single nucleotide polymorphisms (SNPs) on the human Y chromosome based on a multiplexed single nucleotide primer extension. This method has the advantage that several SNPs are typed rapidly and simultaneously. We examined 15 different SNP loci on Y chromosome, M9, M105, M122, M125, M128, M130, SRY465, IMS-JST006241, IMS-JST006841, IMS-JST002611, IMS-JST003305, IMS-JST008425, IMS-JST021354, IMS-JST021355 and IMS-JST055457, in 159 Japanese males. From the typing results of these 15 loci, we found 13 haplotypes. Gene diversity for each locus ranged from 0.025 to 0.486 and the haplotype diversity was estimated to be 0.838. This method could be readily applied for personal identification and paternity testing.
AB - We have developed a new method for typing single nucleotide polymorphisms (SNPs) on the human Y chromosome based on a multiplexed single nucleotide primer extension. This method has the advantage that several SNPs are typed rapidly and simultaneously. We examined 15 different SNP loci on Y chromosome, M9, M105, M122, M125, M128, M130, SRY465, IMS-JST006241, IMS-JST006841, IMS-JST002611, IMS-JST003305, IMS-JST008425, IMS-JST021354, IMS-JST021355 and IMS-JST055457, in 159 Japanese males. From the typing results of these 15 loci, we found 13 haplotypes. Gene diversity for each locus ranged from 0.025 to 0.486 and the haplotype diversity was estimated to be 0.838. This method could be readily applied for personal identification and paternity testing.
KW - Haplotype diversity
KW - Single nucleotide polymorphisms
KW - Single nucleotide primer extension
KW - Y chromosome
UR - http://www.scopus.com/inward/record.url?scp=0036734358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036734358&partnerID=8YFLogxK
U2 - 10.1016/S1344-6223(02)00032-9
DO - 10.1016/S1344-6223(02)00032-9
M3 - Article
C2 - 12935671
AN - SCOPUS:0036734358
VL - 4
SP - 202
EP - 206
JO - Legal Medicine
JF - Legal Medicine
SN - 1344-6223
IS - 3
ER -