Abstract
Tabby (Ta) is a semidominat allele of the locus on the mouse X chromosome, which causes a characteristic coat pattern and developmental defects in endocrinic glands. To establish a method for identifying the X chromosome bearing the Ta allele in early preimplantation embryos, we performed PCR amplification of an X chromosomal microsatellite marker locus from preimplantation embryos obtained from mating between XO female and XY male mice. The microsatellite marker locus was shown to be polymorphic between X chromosomes bearing the Ta and wild-type alleles. The amplification of the marker locus from early preimplantation embryos demonstrated that the Ta locus can be correctly typed by this method from embryos as little as 2 cells.
Original language | English |
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Pages (from-to) | 395-398 |
Number of pages | 4 |
Journal | Experimental Animals |
Volume | 45 |
Issue number | 4 |
DOIs | |
Publication status | Published - Oct 1996 |
Keywords
- Microsatellite
- Tabby
- XO
ASJC Scopus subject areas
- Animal Science and Zoology
- Biochemistry, Genetics and Molecular Biology(all)
- veterinary(all)