Type of inflammation differentially affects expression of interleukin 1β and 6, tumor necrosis factor-α and toll-like receptors in subclinical endometritis in mares

Marta J. Siemieniuch, Anna Z. Szóstek, Katarzyna Gajos, Roland Kozdrowski, Marcin Nowak, Kiyoshi Okuda

Research output: Contribution to journalArticle

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Abstract

Mares that fail to conceive or lose their embryos, without showing typical signs of clinical endometritis, should be suspected of subclinical endometritis (SE). In this study, the question was addressed: does SE fully activate selected mechanisms of innate immunity in mares? For this aim, expression of mRNAs for Toll-like Receptor 2 and 4 (TLR 2/4), interleukin 1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor α (TNF) was examined in control mares versus either mares suffering from chronic endometritis (ChE) or subacute suppurative endometritis (SSE). The concentrations of IL-1β, IL-6 and TNF-α in supernatants from endometrial tissue cultures after 4 h incubation were measured using the enzyme immunoassay (EIA) method. Eighty-two warmblood mares, of known breeding history, were enrolled in this study. Based on histopathological assessment, mares were classified as suffering from ChE, SSE or as being healthy. In addition, immuno-localization of both TLR2 and TLR4 as well as TNF-α was investigated in the equine endometria. The mRNA expression of TLR2 (P <0.01), IL-1β (P <0.0001), IL-6 (P <0.0001) and TLR4 and TNF (P <0.05) was up-regulated in endometria of mares suffering from SSE compared with unaffected mares. Concentrations of IL-6 and TNF-α were increased only in mares exhibiting SSE, compared with unaffected (P <0.01 for both) and ChE mares (P <0.05 for both). Immuno-localization of TNF-α and TLRs was confirmed, both in unaffected and SE-affected endometria, and was present in the luminal and glandular epithelia and stromal cells. The severity of inflammation impacts the immune response and fosters activation of innate immunity mechanisms, as observed in the endometria of mares. The intracellular localization of TLRs and TNF-α in the endometria indicates a key role of endometrial epithelial and stromal cells in the immune response and inflammation.

Original languageEnglish
Article numbere0154934
JournalPLoS One
Volume11
Issue number5
DOIs
Publication statusPublished - May 1 2016

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Endometritis
tumor necrosis factors
Toll-Like Receptors
endometritis
interleukin-1
Interleukin-1
interleukin-6
mares
Interleukin-6
Tumor Necrosis Factor-alpha
inflammation
Inflammation
endometrium
Endometrium
stromal cells
Stromal Cells
Toll-Like Receptor 2
Tissue culture
Innate Immunity
Messenger RNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Type of inflammation differentially affects expression of interleukin 1β and 6, tumor necrosis factor-α and toll-like receptors in subclinical endometritis in mares. / Siemieniuch, Marta J.; Szóstek, Anna Z.; Gajos, Katarzyna; Kozdrowski, Roland; Nowak, Marcin; Okuda, Kiyoshi.

In: PLoS One, Vol. 11, No. 5, e0154934, 01.05.2016.

Research output: Contribution to journalArticle

Siemieniuch, Marta J. ; Szóstek, Anna Z. ; Gajos, Katarzyna ; Kozdrowski, Roland ; Nowak, Marcin ; Okuda, Kiyoshi. / Type of inflammation differentially affects expression of interleukin 1β and 6, tumor necrosis factor-α and toll-like receptors in subclinical endometritis in mares. In: PLoS One. 2016 ; Vol. 11, No. 5.
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