Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and is required for surface motility and virulence

Linh Chi Nguyen, Fumiko Taguchi, Quang Minh Tran, Kana Naito, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Hiroshi Ono, Mitsuru Yoshida, Kazuhiro Chiku, Tadashi Ishii, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Type IV pilin (PilA) is a major constituent of pilus and is required for bacterial biofilm formation, surface motility and virulence. It is known that mature PilA is produced by cleavage of the short leader sequence of the pilin precursor, followed by methylation of N-terminal phenylalanine. The molecular mass of the PilA mature protein from the tobacco bacterial pathogen Pseudomonas syringae pv. tabaci 6605 (Pta 6605) has been predicted to be 12329Da from its deduced amino acid sequence. Previously, we have detected PilA as an approximately 13-kDa protein by immunoblot analysis with anti-PilA-specific antibody. In addition, we found the putative oligosaccharide-transferase gene tfpO downstream of pilA. These findings suggest that PilA in Pta 6605 is glycosylated. The defective mutant of tfpO (ΔtfpO) shows reductions in pilin molecular mass, surface motility and virulence towards host tobacco plants. Thus, pilin glycan plays important roles in bacterial motility and virulence. The genetic region around pilA was compared among P.syringae pathovars. The tfpO gene exists in some strains of pathovars tabaci, syringae, lachrymans, mori, actinidiae, maculicola and P.savastanoi pv. savastanoi. However, some strains of pathovars tabaci, syringae, glycinea, tomato, aesculi and oryzae do not possess tfpO, and the existence of tfpO is independent of the classification of pathovars/strains in P.syringae. Interestingly, the PilA amino acid sequences in tfpO-possessing strains show higher homology with each other than with tfpO-nonpossessing strains. These results suggest that tfpO and pilA might co-evolve in certain specific bacterial strains.

Original languageEnglish
Pages (from-to)764-774
Number of pages11
JournalMolecular Plant Pathology
Volume13
Issue number7
DOIs
Publication statusPublished - Sep 2012

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Pseudomonas syringae pv. tabaci
Fimbriae Proteins
Pseudomonas syringae
motility
virulence
Virulence
tobacco
amino acid
Tobacco
Amino Acid Sequence
protein
gene
methylation
homology
Morus
cleavage
antibody
Bacterial Proteins
biofilm
Lycopersicon esculentum

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Soil Science
  • Molecular Biology

Cite this

Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and is required for surface motility and virulence. / Nguyen, Linh Chi; Taguchi, Fumiko; Tran, Quang Minh; Naito, Kana; Yamamoto, Masanobu; Ohnishi-Kameyama, Mayumi; Ono, Hiroshi; Yoshida, Mitsuru; Chiku, Kazuhiro; Ishii, Tadashi; Inagaki, Yoshishige; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Molecular Plant Pathology, Vol. 13, No. 7, 09.2012, p. 764-774.

Research output: Contribution to journalArticle

Nguyen, LC, Taguchi, F, Tran, QM, Naito, K, Yamamoto, M, Ohnishi-Kameyama, M, Ono, H, Yoshida, M, Chiku, K, Ishii, T, Inagaki, Y, Toyoda, K, Shiraishi, T & Ichinose, Y 2012, 'Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and is required for surface motility and virulence', Molecular Plant Pathology, vol. 13, no. 7, pp. 764-774. https://doi.org/10.1111/j.1364-3703.2012.00789.x
Nguyen, Linh Chi ; Taguchi, Fumiko ; Tran, Quang Minh ; Naito, Kana ; Yamamoto, Masanobu ; Ohnishi-Kameyama, Mayumi ; Ono, Hiroshi ; Yoshida, Mitsuru ; Chiku, Kazuhiro ; Ishii, Tadashi ; Inagaki, Yoshishige ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Ichinose, Yuki. / Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and is required for surface motility and virulence. In: Molecular Plant Pathology. 2012 ; Vol. 13, No. 7. pp. 764-774.
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AU - Naito, Kana

AU - Yamamoto, Masanobu

AU - Ohnishi-Kameyama, Mayumi

AU - Ono, Hiroshi

AU - Yoshida, Mitsuru

AU - Chiku, Kazuhiro

AU - Ishii, Tadashi

AU - Inagaki, Yoshishige

AU - Toyoda, Kazuhiro

AU - Shiraishi, Tomonori

AU - Ichinose, Yuki

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