Type IV collagen induces expression of thrombospondin-1 that is mediated by integrin α1β1 in astrocytes

Tomoko Yonezawa, Shunji Hattori, Junko Inagaki, Masae Kurosaki, Tomoyuki Takigawa, Satoshi Hirohata, Toru Miyoshi, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Following brain injury, thrombospondin-1 (TSP-1) is involved in angiogenesis and synaptic recovery. In this study, we used a cold injury-model and found that TSP-1 mRNA was markedly upregulated after brain injury. Immunohistochemistry showed that TSP-1 was upregulated in both the core of the lesion and in the perilesional area of injured brain tissue. Numerous astrocytes immunopositive for glial fibrillary acidic protein (GFAP) were found in the perilesional area, and TSP-1 was also expressed in almost all astrocytes surrounding blood vessels at 4 days after injury. Next, we examined the influence of vascular basement membrane components on TSP-1 expression. When astrocytes were cultured on type IV collagen, TSP-1 was significantly upregulated compared with the expression when cells were grown on laminin, fibronectin, or poly-L-lysine. This increase occurred exclusively when astrocytes were grown on the native form of type IV collagen but not on the heat-denatured form or the non-collagenous 1 domain. Further, integrin a1 and b1 mRNAs were upregulated concomitantly with GFAP mRNA, and integrin a1 protein was localized to the endfeet of astrocytes that surrounded blood vessels in the injured brain. Using function-blocking antibodies, we found that the effect of type IV collagen was attributed to integrin a1b1 in primary astrocytes. Collectively, our results suggest that vascular basement membrane components substantially impact gene expression in astrocytes during brain tissue repair.

Original languageEnglish
Pages (from-to)755-767
Number of pages13
JournalGLIA
Volume58
Issue number7
DOIs
Publication statusPublished - May 2010

Fingerprint

Thrombospondin 1
Collagen Type IV
Integrins
Astrocytes
Blood Vessels
Glial Fibrillary Acidic Protein
Basement Membrane
Brain Injuries
Messenger RNA
Brain
Blocking Antibodies
Laminin
Fibronectins
Lysine
Hot Temperature
Immunohistochemistry
Gene Expression
Wounds and Injuries

Keywords

  • Basement membrane
  • Cold injury
  • Extracellular matrix

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Type IV collagen induces expression of thrombospondin-1 that is mediated by integrin α1β1 in astrocytes. / Yonezawa, Tomoko; Hattori, Shunji; Inagaki, Junko; Kurosaki, Masae; Takigawa, Tomoyuki; Hirohata, Satoshi; Miyoshi, Toru; Ninomiya, Yoshifumi.

In: GLIA, Vol. 58, No. 7, 05.2010, p. 755-767.

Research output: Contribution to journalArticle

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