Abstract
In the present study, we have analyzed the α(IV) chain distribution in the subepithelial basement membrane (BM) of the rat pulmonary airway from the bronchi to alveoli. We have furthermore analyzed the α(IV) chain distribution in the subepithelial BM of the bronchioalveolar duct junction (BADJ) using α(IV) chain specific monoclonal antibodies. Our results show that the BM of the bronchial and bronchiolar epithelium contains [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) molecules and confirmed that the alveolar BM consists of [ α1(IV)]2 α2(IV) and α3(IV) α4(IV) α5(IV) molecules. There are also small regions in BADJ consisting of only [ α1(IV)]2 α2(IV) molecules without α3(IV) α4(IV) α5(IV) and [ α5(IV)]2 α6(IV) molecules. Moreover, the bronchioalveolar stem cells (BASCs) - primordial cells for bronchiolar Clara cells and alveolar type II (AT2) cells - lie adjacent to such small regions. These findings suggest that [α1(IV)]2 α2(IV) may be important for the BASCs to self-renew or to self-maintain themselves and that microenvironments produced by α(IV) chains may be important for cell differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 185-194 |
| Number of pages | 10 |
| Journal | Archives of Histology and Cytology |
| Volume | 71 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 |
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ASJC Scopus subject areas
- Histology
Cite this
Type IV collagen alpha chains of the basement membrane in the rat bronchioalveolar transitional segment. / Hinenoya, Noriko; Naito, Ichiro; Momota, Ryusuke; Sado, Yoshikazu; Kumagishi, Kanae; Ninomiya, Yoshifumi; Ohtsuka, Aiji.
In: Archives of Histology and Cytology, Vol. 71, No. 3, 2008, p. 185-194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Type IV collagen alpha chains of the basement membrane in the rat bronchioalveolar transitional segment
AU - Hinenoya, Noriko
AU - Naito, Ichiro
AU - Momota, Ryusuke
AU - Sado, Yoshikazu
AU - Kumagishi, Kanae
AU - Ninomiya, Yoshifumi
AU - Ohtsuka, Aiji
PY - 2008
Y1 - 2008
N2 - In the present study, we have analyzed the α(IV) chain distribution in the subepithelial basement membrane (BM) of the rat pulmonary airway from the bronchi to alveoli. We have furthermore analyzed the α(IV) chain distribution in the subepithelial BM of the bronchioalveolar duct junction (BADJ) using α(IV) chain specific monoclonal antibodies. Our results show that the BM of the bronchial and bronchiolar epithelium contains [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) molecules and confirmed that the alveolar BM consists of [ α1(IV)]2 α2(IV) and α3(IV) α4(IV) α5(IV) molecules. There are also small regions in BADJ consisting of only [ α1(IV)]2 α2(IV) molecules without α3(IV) α4(IV) α5(IV) and [ α5(IV)]2 α6(IV) molecules. Moreover, the bronchioalveolar stem cells (BASCs) - primordial cells for bronchiolar Clara cells and alveolar type II (AT2) cells - lie adjacent to such small regions. These findings suggest that [α1(IV)]2 α2(IV) may be important for the BASCs to self-renew or to self-maintain themselves and that microenvironments produced by α(IV) chains may be important for cell differentiation.
AB - In the present study, we have analyzed the α(IV) chain distribution in the subepithelial basement membrane (BM) of the rat pulmonary airway from the bronchi to alveoli. We have furthermore analyzed the α(IV) chain distribution in the subepithelial BM of the bronchioalveolar duct junction (BADJ) using α(IV) chain specific monoclonal antibodies. Our results show that the BM of the bronchial and bronchiolar epithelium contains [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) molecules and confirmed that the alveolar BM consists of [ α1(IV)]2 α2(IV) and α3(IV) α4(IV) α5(IV) molecules. There are also small regions in BADJ consisting of only [ α1(IV)]2 α2(IV) molecules without α3(IV) α4(IV) α5(IV) and [ α5(IV)]2 α6(IV) molecules. Moreover, the bronchioalveolar stem cells (BASCs) - primordial cells for bronchiolar Clara cells and alveolar type II (AT2) cells - lie adjacent to such small regions. These findings suggest that [α1(IV)]2 α2(IV) may be important for the BASCs to self-renew or to self-maintain themselves and that microenvironments produced by α(IV) chains may be important for cell differentiation.
UR - http://www.scopus.com/inward/record.url?scp=59949086411&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59949086411&partnerID=8YFLogxK
U2 - 10.1679/aohc.71.185
DO - 10.1679/aohc.71.185
M3 - Article
C2 - 19194041
AN - SCOPUS:59949086411
VL - 71
SP - 185
EP - 194
JO - Archives of Histology and Cytology
JF - Archives of Histology and Cytology
SN - 0914-9465
IS - 3
ER -