Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice

Aiko Ishiwata, Misa Ozawa, Hiroshi Nagasaki, Makio Kato, Yusaku Noda, Takahiro Yamaguchi, Misuzu Nosaka, Sae Shimizu-Sato, Akie Nagasaki, Masahiko Maekawa, Hiro Yuki Hirano, Yutaka Sato

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35 Citations (Scopus)

Abstract

Leaf shape is one of the key determinants of plant architecture. Leaf shape also affects the amount of sunlight captured and influences photosynthetic efficiency; thus, it is an important agronomic trait in crop plants. Understanding the molecular mechanisms governing leaf shape is a central issue of plant developmental biology and agrobiotechnology. Here, we characterized the narrow-leaf phenotype of FL90, a linkage tester line of rice (Oryza sativa). Light and scanning electron microscopic analyses of FL90 leaves revealed defects in the development of marginal regions and a reduction in the number of longitudinal veins. The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS. The overexpression of NAL2/3 in transgenic rice plants results in wider leaves containing increased numbers of veins, suggesting that NAL2/3 expression regulates leaf width. Thus, NAL2/3 can be used to modulate leaf shape and improve agronomic yield in crop plants.

Original languageEnglish
Pages (from-to)779-792
Number of pages14
JournalPlant and Cell Physiology
Volume54
Issue number5
DOIs
Publication statusPublished - May 1 2013

Keywords

  • Leaf width
  • Mutant
  • NAL
  • Narrow leaf
  • Oryza sativa
  • WOX

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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    Ishiwata, A., Ozawa, M., Nagasaki, H., Kato, M., Noda, Y., Yamaguchi, T., Nosaka, M., Shimizu-Sato, S., Nagasaki, A., Maekawa, M., Hirano, H. Y., & Sato, Y. (2013). Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice. Plant and Cell Physiology, 54(5), 779-792. https://doi.org/10.1093/pcp/pct032